Structure of PDB 6mes Chain A

Receptor sequence

>6mesA (length=521) Species: 185431 (Trypanosoma brucei brucei TREU927)

VEKVFFVTSPIYYVNAAPHIGHVYSTLITDVIGRYHRVKGERVFALTGTD
EHGQKVAEAAKQKQVSPYDFTTAVAGEFKKCFEQMDYSIDYFIRTTNEQH
KAVVKELWTKLEQKGDIYLGRYEGWYSISDESFLTPQNITDGVDKDGNPC
KVSLESGHVVTWVSEENYMFRLSAFRERLLEWYHANPGCIVPEFRRREVI
RAVEKGLPDLSVSRARATLHNWAIPVPGNPDHCVYVWLDALTNYLTGSRL
RVDESGKEVSLVDDFNELERFPADVHVIGKDILKFHAIYWPAFLLSAGLP
LPKKIVAHGWWTKNVFDPVEKAEEFGYDALKYFLLRESGFSDDGDYSDKN
MIARLNGELADTLGNLVMRCTSAKINVNGEWPSPAAYTEEDESLIQLIKD
LPGTADHYYLIPDIQKAIIAVFDVLRAINAYVTDMAPWKLVKTDPERLRT
VLYITLEGVRVTTLLLSPILPRKSVVIFDMLGVPEVHRKGIENFEFGAVP
PGTRLGPAVEGEVLFSKRSTE

3D structure

• PDB: 6mes Structure-guided discovery of selective methionyl-tRNA synthetase inhibitors with potent activity against Trypanosoma brucei
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): H256 H259 S364 D367 S390 S393
• Catalytic site (residue number reindexed from 1): H19 H22 S127 D130 S153 S156
• Enzyme Commision number: 6.1.1.10: methionine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MET	A	P247 I248 Y249 Y250 D287 W474 L478 H523 K550	P10 I11 Y12 Y13 D50 W237 L241 H286 K313

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004825 methionine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006431 methionyl-tRNA aminoacylation

External links

• PDB: RCSB:6mes, PDBe:6mes, PDBj:6mes
• PDBsum: 6mes
• UniProt: Q38C91