Structure of PDB 6mcc Chain A

Receptor sequence

>6mccA (length=1364) Species: 160490 (Streptococcus pyogenes M1 GAS)

KKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLL
FKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIK
DKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLK
RRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSL
TFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMG
RHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVE
NTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSI
DNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTK
AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIRE
VKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYP
KLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITL
ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQT
GGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS
LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI
REQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSI
TGLYETRIDLSQLG

3D structure

• PDB: 6mcc Temperature-Responsive Competitive Inhibition of CRISPR-Cas9.
• Chain: A
• Resolution: 3.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K30 L35 K44 N46 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 R78 L101 S104 F105 R115 H116 H129 E130 T134 I135 H160 K163 F164 R165 G166 H167 Y325 H328 R340 K348 F351 F352 Y359 Q402 R403 N407 F446 I448 Y450 L455 R457 N459 T472 I473 M495 N497 R661 A728 G729 S730 K749 A764 R765 R919 T924 Q926 M1089 P1090 Q1091 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 Y1131 F1134 M1169 E1225 L1226 A1227 H1349 Q1350 S1351 Y1356	K28 L33 K42 N44 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 R76 L99 S102 F103 R113 H114 H127 E128 T132 I133 H158 K161 F162 R163 G164 H165 Y323 H326 R338 K346 F349 F350 Y357 Q400 R401 N405 F444 I446 Y448 L453 R455 N457 T470 I471 M493 N495 R659 A726 G727 S728 K747 A762 R763 R917 T922 Q924 M1087 P1088 Q1089 T1100 G1101 G1102 F1103 I1108 P1110 K1111 R1120 K1121 K1122 Y1129 F1132 M1167 E1223 L1224 A1225 H1347 Q1348 S1349 Y1354

Gene Ontology

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UnboundLocalError

Python 3.6.8: /usr/bin/python3 Sat Feb 22 00:19:56 2025

A problem occurred in a Python script. Here is the sequence of function calls leading up to the error, in the order they occurred.

 /var/www/html/BioLiP/pdb.cgi in <module>()

   1443                 pubmed=display_regular_ligand(pdbid,asym_id,lig3,ligIdx,title)

   1444         else:

=> 1445             pubmed,uniprot=display_protein_receptor(pdbid,asym_id,title)

   1446     

   1447     uniprot_line=''

pubmed = '', uniprot = '', display_protein_receptor = <function display_protein_receptor>, pdbid = '6mcc', asym_id = 'A', title = 'Temperature-Responsive Competitive Inhibition of CRISPR-Cas9.'

 /var/www/html/BioLiP/pdb.cgi in display_protein_receptor(pdbid='6mcc', asym_id='A', title='Temperature-Responsive Competitive Inhibition of CRISPR-Cas9.')

    839 

    840     if go:

=>  841         display_go(go,uniprot,pdbid,asym_id)

    842     return pubmed,uniprot

    843 

global display_go = <function display_go>, go = '0003676,0004519', uniprot = '', pdbid = '6mcc', asym_id = 'A'

 /var/www/html/BioLiP/pdb.cgi in display_go(go='0003676,0004519', uniprot='', pdbid='6mcc', asym_id='A')

    480         '''.replace("$namespace_link",namespace_link

    481           ).replace("$namespace",namespace

=>  482           ).replace("$uniprot",u

    483         ))

    484         for l,(term,name) in enumerate(go2aspect[Aspect]):

u undefined

UnboundLocalError: local variable 'u' referenced before assignment
      args = ("local variable 'u' referenced before assignment",)
      with_traceback = <built-in method with_traceback of UnboundLocalError object>