Structure of PDB 6m4m Chain A

Receptor sequence

>6m4mA (length=496) Species: 6396 (Eisenia fetida)

FQYFGSYYCQPGRDVIVHLFEWKWTDIERECQWLADHNYCGVQVSPPNEH
RIVTDPPYPWWQRYQPVSYTMNSRSGSETQFRNMVTTCNNLGVYIYVDGV
LNHMTGGGSGTGSDGNSFDGDSLQYPGVPYGPNDFHGSDDCSTSDGEIHD
YNNPTEVRNCRLSGLRDLDGSANYVRDVEAEFANRLIGWGVAGFRLDACK
HMWPGDLEAILGRLNQLNTQWFPAGRNAYIYQQVIDLGGEAVKATEYTYL
GRVTEFKHGQNLGNVVRKNNGQRLANLVNFGEGWGQLSGFDALVFIDNHD
NQRGHGAGGFGTILTFFEANMYKIATAFELAWDYGHVRLMSSYNWPRNIV
NGVDTNDWVGPPTNGGGDTKDVECFNGEWICEHRWREMYNMVRFHNVAIG
NPVSNWWDNGFQAIAFGRGNRGFIFINNEDFAITQTLQTGLPGGEYCDVI
SCDNNRPPCGNSGGACRATVIVNGDGTATFDVPNGENPMIAIHVFL

3D structure

• PDB: 6m4m X-ray crystallographic structural studies of alpha-amylase I from Eisenia fetida.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.1: alpha-amylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	K216 E256 G322	K200 E240 G306
BS02	GLC	A	A214 H217 Q249	A198 H201 Q233
BS03	GLC	A	W76 Y80 H119 D213 Q249 H315 D316	W60 Y64 H103 D197 Q233 H299 D300
BS04	GLC	A	W76 W77 Q81 H321	W60 W61 Q65 H305
BS05	GLC	A	W77 S179	W61 S163
BS06	GLC	A	E163 S179	E147 S163
BS07	GLC	A	K339 F391 W401 E403	K323 F375 W385 E387
BS08	GLC	A	R400 W401 R402 E403	R384 W385 R386 E387
BS09	CA	A	N118 R174 D183 H217	N102 R158 D167 H201

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004556 alpha-amylase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0043169 cation binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005615 extracellular space

External links

• PDB: RCSB:6m4m, PDBe:6m4m, PDBj:6m4m
• PDBsum: 6m4m
• PubMed: 32876059
• UniProt: A0A173N065