Structure of PDB 6m0x Chain A

Receptor sequence

>6m0xA (length=1032) Species: 322159 (Streptococcus thermophilus LMD-9)

LVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVRRTNRQGR
RLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNE
ELFIALKNMVKHRGISYKTPGQIQLERYQTYGQLRGDFTVEKDGKKHRLI
NVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKRKYYHGPGN
EKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLL
NDLNNLTVPTKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLDVADIKG
YRIDKSGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTERE
GIQEALEHEFADGSFSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELI
PELYETSEEQMTILTRLGYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIK
EYGDFDNIVIEMARETNEDDEKKAIQKIQKANKDEKDAAMLKAANQYNGK
AELPHSVFHGHKQLATKIRLWHQQGERCLYTGKTISIHDLINNSNQFEVD
AILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDSMDDAWSFRELKA
FVRESKTLSNKKKEYLLTEEDISKFDVIERNLVDTRYASRVVLNALQEHF
RAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLN
LPYQHFVDTLKSKEFEDSILFSYQVDSKFNRKISDATIYATRQAKVGKDK
ADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKFLMYRHDPQTFEKVIEPI
LENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPEIKSLKYYD
SKLGNHIDITPKDSNNKVVLQSVSPWRADVYFNKTTGKYEILGLKYADLQ
FEKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQ
QLFRFLSRTMPKQKHYVELKPYDKQKFEGGEALIKVLGNVANSGQCKKGL
GKSNISIYKVRTDVLGNQHIIKNEGDKPKLDF

3D structure

• PDB: 6m0x Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
• Chain: A
• Resolution: 2.561 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Q39 A40 N43 V45 R46 R47 N49 R50 R53 R54 L55 R57 R58 H61 R63 R68 I84 N85 Y89 K110 K114 H115 R116 G117 Y159 G163 Q164 L165 R166 G167 N182 K222 R223 Y225 G228 P229 G230 N231 K233 S234 T236 Y238 R240 F252 T260 K270 N279 R338 K341 H348 H425 N426 F427 Q446 M447 K471 Y478 N479 P480 K484 R487 R512 Q527 N530 K534 R567 D609 S610 L611 E681 R682 V685 R692 Q699 V713 R715 Q813 V814 D815 S816 K817 N819 R820 K821 I822 I827 A829 R831 K848 L873 M874 H877 K922 Y923 S924 K925 N928 P930 E931 K933 S934 L935 K936 P950 D952 S953 N954 V957 L988 Q989 F990 K992 G993 Y997	Q36 A37 N40 V42 R43 R44 N46 R47 R50 R51 L52 R54 R55 H58 R60 R65 I81 N82 Y86 K107 K111 H112 R113 G114 Y128 G132 Q133 L134 R135 G136 N151 K191 R192 Y194 G197 P198 G199 N200 K202 S203 T205 Y207 R209 F221 T229 K239 N248 R302 K305 H312 H389 N390 F391 Q410 M411 K423 Y430 N431 P432 K436 R439 R464 Q479 N482 K486 R519 D561 S562 L563 E629 R630 V633 R640 Q647 V661 R663 Q724 V725 D726 S727 K728 N730 R731 K732 I733 I738 A740 R742 K759 L784 M785 H788 K833 Y834 S835 K836 N839 P841 E842 K844 S845 L846 K847 P861 D863 S864 N865 V868 L899 Q900 F901 K903 G904 Y908
BS02	dna	A	Y120 Y225 F252 L255 G257 R267 N286 Y337 R338 I339 K341 T383 W424 E445 T451 R512 E513 K520 Q524 Q527 K560 K565 E596 V597 D598 S604 N622 Q623 Q627 F673 Y689 R692 D824 A825 T826 Y828 M1049 Q1052 Y1055 A1080 S1082 K1087	Y117 Y194 F221 L224 G226 R236 N255 Y301 R302 I303 K305 T347 W388 E409 T415 R464 E465 K472 Q476 Q479 K512 K517 E548 V549 D550 S556 N574 Q575 Q579 F625 Y637 R640 D735 A736 T737 Y739 M960 Q963 Y966 A991 S993 K998
BS03	dna	A	F673 K867 K941 G943 N944 S961 V962 P964 K984 K1059 Q1084 K1086	F625 K778 K852 G854 N855 S872 V873 P875 K895 K970 Q995 K997
BS04	MG	A	D598 N622	D550 N574
BS05	MG	A	S1082 G1083	S993 G994

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6m0x, PDBe:6m0x, PDBj:6m0x
• PDBsum: 6m0x
• UniProt: Q03LF7|CAS9A_STRTD CRISPR-associated endonuclease Cas9 1 (Gene Name=cas9-1)