Structure of PDB 6lsf Chain A

Receptor sequence

>6lsfA (length=463) Species: 39054 (Enterovirus A71)

GEIQWVKPNKETGRLNINGPTRTKLEPSVFHDVFEGNKEPAVLHSKDPRL
EVDFEQALFSKYVGNTLYEPDEYIKEAALHYANQLKQLDIDTSQMSMEEA
CYGTENLEAIDLHTSAGYPYSALGIKKRDILDSTTRDVSKMKFYMDKYGL
DLPYSTYVKDELRSIDKIKKGKSRLIEASSLNDSVYLRMTFGHLYETFHA
NPGTVTGSAVGCNPDTFWSKLPILLPGSLFAFDYSGYDASLSPVWFRALE
LVLREIGYSEEAVSLVEGINHTHHVYRNKTYCVLGGMPSGMSGTSIFNSM
INNIIIRALLIKTFKGIDLDELNMVAYGDDVLASYPFPIDCLELARTGKE
YGLTMTPADKSPCFNEVNWDNATFLKRGFLPDEQFPFLIHPTMPMKEIHE
SIRWTKDARNTQDHVRSLCLLAWHNGKQEYEKFVSAIRSVPVGKALAIPN
YENLRRNWLELFH

3D structure

• PDB: 6lsf Stringent control of the RNA-dependent RNA polymerase translocation revealed by multiple intermediate structures.
• Chain: A
• Resolution: 2.152 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	P20 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 N213 G290 M291 S292 Y327	P20 T114 S115 K127 Y157 K159 I176 A178 R188 H199 G211 C212 N213 G290 M291 S292 Y327
BS02	rna	A	H113 S133 S295 Y327 L375 K376 S401 N410 D413 H414	H113 S133 S295 Y327 L375 K376 S401 N410 D413 H414
BS03	ZN	A	H271 H273 C282	H271 H273 C282

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:6lsf, PDBe:6lsf, PDBj:6lsf
• PDBsum: 6lsf
• PubMed: 32451382
• UniProt: E5RPG3