Structure of PDB 6l71 Chain A

Receptor sequence

>6l71A (length=296) Species: 9606 (Homo sapiens)

SQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTG
LYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFM
RLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRH
EYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFL
KVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGGMDF
DSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQ

3D structure

• PDB: 6l71 Sirtuin 2 protein with H3K18 myristoylated peptide
• Chain: A
• Resolution: 2.109 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): P94 D95 F96 R97 N168 D170 H187
• Catalytic site (residue number reindexed from 1): P42 D43 F44 R45 N116 D118 H135
• Enzyme Commision number: 2.3.1.-
  2.3.1.286: protein acetyllysine N-acetyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	H187 V233 F234 F235 E237 L239 Q265 V266 Q267	H135 V181 F182 F183 E185 L187 Q213 V214 Q215
BS02	NCA	A	A85 P94 F96 N168 I169	A33 P42 F44 N116 I117	BindingDB: IC50=11000nM
BS03	YOY	A	A85 G86 T89 D95 F96 R97 Q167 I169 H187 F190 F235 G261 T262 S263 N286 K287 E288 E323 C324	A33 G34 T37 D43 F44 R45 Q115 I117 H135 F138 F183 G209 T210 S211 N234 K235 E236 E264 C265
BS04	YDD	A	G84 G86 T89 D95 F96 R97 F119 L138 Q167 H187 F190 G261 T262 S263 N286 K287 E288 E323 C324	G32 G34 T37 D43 F44 R45 F67 L86 Q115 H135 F138 G209 T210 S211 N234 K235 E236 E264 C265

Gene Ontology

Molecular Function

• GO:0017136 NAD-dependent histone deacetylase activity
• GO:0051287 NAD binding
• GO:0070403 NAD+ binding

External links

• PDB: RCSB:6l71, PDBe:6l71, PDBj:6l71
• PDBsum: 6l71
• UniProt: Q8IXJ6|SIR2_HUMAN NAD-dependent protein deacetylase sirtuin-2 (Gene Name=SIRT2)