Structure of PDB 6l66 Chain A

Receptor sequence

>6l66A (length=298) Species: 9606 (Homo sapiens)

GSQKERLLDELTLEGVARYMQSQRCRRVICLVGAGISTSAGIPDFRSPST
GLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYF
MRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCR
HEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDF
LKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGGGM
DFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQ

3D structure

• PDB: 6l66 Sirtuin 2 protein with H3K18 myristoylated peptide
• Chain: A
• Resolution: 2.169 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): P94 D95 F96 R97 N168 D170 H187
• Catalytic site (residue number reindexed from 1): P43 D44 F45 R46 N117 D119 H136
• Enzyme Commision number: 2.3.1.-
  2.3.1.286: protein acetyllysine N-acetyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	E116 H187 V233 F235 G236 E237 Q265 V266 Q267	E65 H136 V182 F184 G185 E186 Q214 V215 Q216
BS02	ZN	A	C195 C221 C224	C144 C170 C173
BS03	NAD	A	G84 A85 G86 T89 P94 D95 F96 R97 Q167 N168 I169 D170 H187 G261 T262 S263 N286 K287 E288 E323 C324	G33 A34 G35 T38 P43 D44 F45 R46 Q116 N117 I118 D119 H136 G210 T211 S212 N235 K236 E237 E266 C267
BS04	3LX	A	I169 F190 V233 F235	I118 F139 V182 F184

Gene Ontology

Molecular Function

• GO:0017136 NAD-dependent histone deacetylase activity
• GO:0051287 NAD binding
• GO:0070403 NAD+ binding

External links

• PDB: RCSB:6l66, PDBe:6l66, PDBj:6l66
• PDBsum: 6l66
• UniProt: Q8IXJ6|SIR2_HUMAN NAD-dependent protein deacetylase sirtuin-2 (Gene Name=SIRT2)