Structure of PDB 6kwq Chain A

Receptor sequence

>6kwqA (length=462) Species: 39054 (Enterovirus A71)

GEIQWVKPNKETGRLNINGPTRTKLEPSVFHDIFEGNKEPAVLHSKDPRL
EVDFEQALFSKYVGNTLHEPDEYIKEAALHYANQLKQLEINTSQMSMEEA
CYGTENLEAIDLHTSAGYPYSALGIKKRDILDPTTRDVSKMKFYMDKYGL
DLPYSTYVKDELRSIDKIKKGKSRLIEASSLNDSVYLRMAFGHLYEAFHA
NPGTITGSAVGCNPDTFWSKLPILLPGSLFAFDYSGYDASLSPVWFRALE
LVLREIGYSEEAVSLIEGINHTHHVYRNKTYCVLGGMPSGCSGTSIFNSM
INNIIIRALLIKTFKGIDLDELNMVAYGDDVLASYPFPIDCLELAKTGKE
YGLTMTPADKSPCFNEVNWGNATFLKRGFLPDEQFPFLIHPTMPMREIHE
SIRWTKDARNTQDHVRSLCLLAWHNGKQEYEKFVSTIRSVPVGRALAIPN
YENLRRNWLELF

3D structure

• PDB: 6kwq A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability.
• Chain: A
• Resolution: 1.76 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	N18 P20 T21 K24 H44 S45 K46 E108 T114 S115 K126 K127 Y157 K159 I176 A178 R188 H199 G211 C212 N213 R277 S289 G290 C291 S292 Y327 R416 L420	N18 P20 T21 K24 H44 S45 K46 E108 T114 S115 K126 K127 Y157 K159 I176 A178 R188 H199 G211 C212 N213 R277 S289 G290 C291 S292 Y327 R416 L420	PDBbind-CN: Kd=0.18uM
BS02	rna	A	H113 R128 Y327 G328 D330 L375 K376 S401 K406 N410 D413 H414 S417	H113 R128 Y327 G328 D330 L375 K376 S401 K406 N410 D413 H414 S417	PDBbind-CN: Kd=0.18uM
BS03	ZN	A	H271 H273 C282	H271 H273 C282

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:6kwq, PDBe:6kwq, PDBj:6kwq
• PDBsum: 6kwq
• PubMed: 31863580
• UniProt: M4QLY4