Structure of PDB 6kse Chain A

Receptor sequence

>6kseA (length=607) Species: 1773 (Mycobacterium tuberculosis)

MSHYRSNVRDQVFNLFEVLGVDKALGHGEFSDVDVDTARDMLAEVSRLAE
GPVAESFVEGDRNPPVFDPKTHSVMLPESFKKSVNAMLEAGWDKVGIDEA
LGGMPMPKAVVWALHEHILGANPAVWMYAGGAGFAQILYHLGTEEQKKWA
VLAAERGWGSTMVLTEPDAGSDVGAARTKAVQQADGSWHIDGVKRFITSG
DSGDLFENIFHLVLARPEGAGPGTKGLSLYFVPKFLFDVETGEPGERNGV
FVTNVEHKMGLKVSATCELAFGQHGVPAKGWLVGEVHNGIAQMFEVIEQA
RMMVGTKAIATLSTGYLNALQYAKSRVQGADLTQMTDKTAPRVTITHHPD
VRRSLMTQKAYAEGLRALYLYTATFQDAAVAEVVHGVDAKLAVKVNDLML
PVVKGVGSEQAYAKLTESLQTLGGSGFLQDYPIEQYIRDAKIDSLYAGTT
AIQAQDFFFRKIVRDKGVALAHVSGQIQEFVDSGRLKTERALLAKALTDV
QGMAAALTGYLMAAQQDVTSLYKVGLGSVRFLMSVGDLIIGWLLQRQAAV
AVAALDAGATGDERSFYEGKVAVASFFAKNFLPLLTSTREVIETLDNDIM
ELDEAAF

3D structure

• PDB: 6kse Structural basis for the broad substrate specificity of two acyl-CoA dehydrogenases FadE5 from mycobacteria.
• Chain: A
• Resolution: 1.998 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L164 T165 A300 R460
• Catalytic site (residue number reindexed from 1): L164 T165 A300 R460
• Enzyme Commision number: 1.3.8.1: short-chain acyl-CoA dehydrogenase.
  1.3.8.7: medium-chain acyl-CoA dehydrogenase.
  1.3.8.8: long-chain-acyl-CoA dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	M162 L164 T165 G170 S171 F196 T198 I442 Y446 T449	M162 L164 T165 G170 S171 F196 T198 I442 Y446 T449
BS02	ST9	A	D93 H115 W126 G130 G133 F134 M162 S171 T224 K225 I290 F294 A300 R301 Y446 A447 I452 D456 R460 K461	D93 H115 W126 G130 G133 F134 M162 S171 T224 K225 I290 F294 A300 R301 Y446 A447 I452 D456 R460 K461
BS03	FAD	A	R326 Q328 I345 H348 Q420 T421 G423 G424	R326 Q328 I345 H348 Q420 T421 G423 G424

Gene Ontology

Molecular Function

• GO:0004466 long-chain fatty acyl-CoA dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
• GO:0016937 short-chain fatty acyl-CoA dehydrogenase activity
• GO:0070991 medium-chain fatty acyl-CoA dehydrogenase activity

Biological Process

• GO:0006631 fatty acid metabolic process
• GO:0052572 response to host immune response

Cellular Component

• GO:0005576 extracellular region
• GO:0005886 plasma membrane

External links

• PDB: RCSB:6kse, PDBe:6kse, PDBj:6kse
• PDBsum: 6kse
• PubMed: 32601219
• UniProt: O53666|FADE5_MYCTU Broad-specificity linear acyl-CoA dehydrogenase FadE5 (Gene Name=fadE5)