Structure of PDB 6kc8 Chain A

Receptor sequence

>6kc8A (length=1036) Species: 604162 (Neisseria meningitidis 8013)

SINYILGLDIGIASVGWAMVEIDEEENPIRLIDLGVRVFERAEVPGDSLA
MARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIKSLPNT
PWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNETGDFRTPAELAL
NKFEKESGHIRNQRSDYSHTFSRKDLQAELILLFEKQKEFGNPHVSGGLK
EGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAERFIWLTK
LNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFF
KGLRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEI
GTAFSLFKTDEDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIV
PLMEQGKRYDEACAEIYGDHYGKKNTEEKIYLPPIPADEIRNPVVLRALS
QARKVINGVVRRYGSPARIHIETAREVGKSFKDRKEIEKRQEENRKDREK
AAAKFREYFSKDILKLRLYEQQHGKCLYSGKEINLGRLNEKGYVEIDHAL
PFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFNGKDNSREWQEFKARVE
TSRFPRSKKQRILLQDGFKERNLNDTRYVNRFLCQFVADRMRLTGKGKKR
VFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQKITRF
VRYKEMNAFDGKTIDTGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPE
FEEADTLEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMET
VKSAKRLDEGVSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHK
DDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKTGVWVRNHNGIADNAT
MVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGKDEEDWQLIDDSFN
FKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIRIHDLDHKIGKNGI
LEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR

3D structure

• PDB: 6kc8 Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R48 A59 R62 R63 R66 R69 R70 L71 R73 R74 H77 R78 R81 R84 R88 N108 P110 W111 H133 K136 H137 R138 G139 Y140 G190 H191 I192 R193 N194 Q195 R196 R205 Q242 R243 A245 K253 T259 F277 Q422 Y441 H452 Y453 R473 N474 P475 R479 S482 K486 R507 N526 R527 R530 N666 N668 R675 Q679 Q738 Q739 T742 R746 Y747 R836 M838 G840 Q841 G842 H843 V847 S849 K851 L860 R861 V876 N877 R880 E881 K909 T917 Q918 Q919 K921 A922 V923 R924 R936 N937 N939 R949 S966 W967 A970 K971 Q1062 R1071 C1073 R1074 K1076 V1081 R1082	R41 A50 R53 R54 R57 R60 R61 L62 R64 R65 H68 R69 R72 R75 R79 N99 P101 W102 H124 K127 H128 R129 G130 Y131 G158 H159 I160 R161 N162 Q163 R164 R173 Q210 R211 A213 K221 T227 F245 Q390 Y409 H420 Y421 R441 N442 P443 R447 S450 K454 R475 N494 R495 R498 N622 N624 R631 Q635 Q694 Q695 T698 R702 Y703 R790 M792 G794 Q795 G796 H797 V801 S803 K805 L814 R815 V830 N831 R834 E835 K863 T871 Q872 Q873 K875 A876 V877 R878 R890 N891 N893 R903 S920 W921 A924 K925 Q1016 R1025 C1027 R1028 K1030 V1035 R1036
BS02	dna	A	Y140 R144 L246 M254 L255 G256 K266 N285 K333 K390 D418 K419 F420 R440 D442 H452 Y453 R473 A506 R507 E508 R516 K517 Q523 R671 Y672 R675 G691 K692 A736 Q739	Y131 R135 L214 M222 L223 G224 K234 N253 K301 K358 D386 K387 F388 R408 D410 H420 Y421 R441 A474 R475 E476 R484 K485 Q491 R627 Y628 R631 G647 K648 A692 Q695
BS03	dna	A	N944 T946 M947 Y965 H1024 R1025	N898 T900 M901 Y919 H978 R979
BS04	dna	A	M844 E845 T846 Q981 K1013 N1029 G1049 I1050 G1051 K1053 T1054	M798 E799 T800 Q935 K967 N983 G1003 I1004 G1005 K1007 T1008

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6kc8, PDBe:6kc8, PDBj:6kc8
• PDBsum: 6kc8
• PubMed: 31668930
• UniProt: C9X1G5|CAS9_NEIM8 CRISPR-associated endonuclease Cas9 (Gene Name=cas9)