Structure of PDB 6kc0 Chain A

Receptor sequence

>6kc0A (length=674) Species: 83332,523850

MEIDELTALGGLLHDIGKPVQRAGLYSGDHSTQGARFLRDLAENTGRAEY
ELLSLFSEFHNDELMIRRIKELSPERFGLTMEDVLNALWIVYEADNLAPQ
ASRPLYSVFNPGKAYPWAELDFEKELPVPGDVFSIRSQDYRELVKRLWEE
LSKAKLRSDRLLPVLEKYLTFVSSVTSEGNIISLYDHMRMTSAIALAMLR
AGCTAEDVRSGRCRKEKRFLLIEGDFSGIQDFIYRVSGKGTLKYLRARSA
YLELIGWDVVLEILSRLGLTRANVVFNAGGHFMIIAQNTPDAVKELEEIR
AKAVEWLYREFESDLYLAIEWEPVSGREFGREGGKNLFAEARKRLKHKLT
VRKLKRFCPVCGRCPTCNRLVSLGGNLPKLLGFGRTAKNDAGVLVEGPFS
GFVPYLQGGRPVGEQILVKNTLNPGEIPESAQFVPYFVADYFKARLGVLR
LDVDNLGQAFTHGFGKFNTISRTAAFSRMLSLFFRQHINYVLARPKLRPI
TGDDPARPREATIIYSGGDDVFVVGAWDDVIEFGIELRERFHEFTQGKLT
VSAGIGMFPDKYPISVMAREVGDLEDAAKSLPGKNGVALFDREFTFGWDE
LLSKVIEEKYRHIADYFSGNEERGMAFIYKLLEWVYFLTPFQQFANRLHQ
WFQDPTDAKQLKTALHLYIYRTRK

3D structure

• PDB: 6kc0 Mycobacterium tuberculosis CRISPR/Cas system Csm1 holds clues to the evolutionary relationship between DNA polymerase and cyclase activity.
• Chain: A
• Resolution: 2.295 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	S241 I243 Q244 S263 L266 G293 K367 Y623 D628	S227 I229 Q230 S249 L252 G279 K353 Y515 D520
BS02	ATP	A	A292 H295 V556 D557 N558 L559 G560 F563 S585 G626 D627 K692	A278 H281 V453 D454 N455 L456 G457 F460 S477 G518 D519 K584
BS03	MG	A	D555 V556 D627	D452 V453 D519
BS04	MG	A	D555 D627	D452 D519

External links

• PDB: RCSB:6kc0, PDBe:6kc0, PDBj:6kc0
• PDBsum: 6kc0
• PubMed: 33352158
• UniProt: B6YWB8|CAS10_THEON CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A) (Gene Name=csm1);
  P71629