Structure of PDB 6k35 Chain A

Receptor sequence

>6k35A (length=640) Species: 669 (Vibrio harveyi)

GSEYRVDLVVLSEQKQNCRFGLTFHNLSDQDLNSWGLTFAFDRYILPDSV
SNGQLTQIGSFCTLKPEGIVLAANHHYYCEFSIGSNPFRYYSDGFNEAMI
DFVVDGQPQRAQVDVTPIVLASPYRERSDIPASLTHAQPLLPKPNHIEVS
DHSFTFDEQAGVAIYTDLANSAKAWLLEELQRIHQFTLSSSNSGKIIFKS
NPTLDEGAYKLKVSEESIKIEAGSSSGFTHACATLLQLLKRDEATKTMEA
VCCSIIDSPRFRYRGMMLDCARHFHSVEQVKRLINLLAHYKLNTFHWHLT
DDEGWRVEIKSLPQLTEIGAWRGIDETIEPQYTHLSQRYGGFYTQEEIRD
VIAFAEQRGITIIPEIDVPGHCRAAIKSLPHLLIEAEDTTEYRSIQHYND
NVINPALPGSYEFIDKVLEEIAALFPAPYVHIGADEVPNGVWSKSPACQA
LMEQLGYTDYKELQGHFLRHAEDKLRKLGKRMLGWEEAQHGNKVSKDTVI
YSWLSEEAALNCARQGFDVVLQPAQTTYLDMTQDYAPEEPGVDWANPLPL
EKAYNYEPLAEVPADDPIRKRIWGIQTALWCEIINNPSRMDYMIFPRLTA
MAEACWTEKQHRDWTDYLSRLKGHLPLLDLQGVNYRKPWK

3D structure

• PDB: 6k35 NAG-thiazoline is a potent inhibitor of the Vibrio campbellii GH20 beta-N-Acetylglucosaminidase.
• Chain: A
• Resolution: 2.36 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D437 E438
• Catalytic site (residue number reindexed from 1): D435 E436
• Enzyme Commision number: 3.2.1.52: beta-N-acetylhexosaminidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NGT	A	R274 D437 E438 W487 W505 Y530 D532 W546 W582 E584	R272 D435 E436 W485 W503 Y528 D530 W544 W580 E582	MOAD: ic50=11.9uM

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0004563 beta-N-acetylhexosaminidase activity
• GO:0016231 beta-N-acetylglucosaminidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0030203 glycosaminoglycan metabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:6k35, PDBe:6k35, PDBj:6k35
• PDBsum: 6k35
• PubMed: 32145141
• UniProt: D9ISE0