Structure of PDB 6jfj Chain A

Receptor sequence

>6jfjA (length=646) Species: 343517 (Paenibacillus barengoltzii)

HGTIDYGDVTVTGGISVFDAKFDELYVYDGDDLGAVYAPEETRFRLWAPT
ASEAFVVLYETEDGMPVKELPMKRDVQGTWTLTVAEDCGGLFYTYRVKVG
EQWNEAVDPYAKAVGVNGTKTAILDLRSTNPEGWENDQKPPLASPTDAVI
YELHVRDLSIHPQSGIREKGKFLGLTEEGTRGPNGIPTGLDHITGLGVTH
VQLLPIYDYSQESVDESRLDEPHYNWGYDPQNYNVPEGSYSTDPHNPAAR
ILELKRLIQKLHARGLRVIMDVVYNHVYDGYLIHFTKLVPGYYLRYKADR
TFSDGTFCGNECASERPIMRKYIIESILHWVREYHIDGFRFDLMGMIDIE
TMNEIRRRLDEIDPTILTIGEGWMMETVLPKELRANQDNAEKLPGIGMFN
DGMRDAVKGDIFIFDRKGFISGGDGFEDGVKRGVAGGINYGGQLRQFAVE
PVQSVNYVECHDNHTLWDKIELSTPGASDEERRAMHRLASAIVLTSQGIP
FLHAGQEFMRTKGGVENSYKSPIEVNWLDWERCAAHQDDVSYMRSLIALR
KAHPAFRLKTADEIRAHLRFEAAPPHTVAFTLRDHAGGDPDRHLYVLYNA
NPGALSLELPALGPWEVRFGGEHVLALEAARLEVRGVGVVVLAVPR

3D structure

• PDB: 6jfj Structural basis of carbohydrate binding in domain C of a type I pullulanase from Paenibacillus barengoltzii.
• Chain: A
• Resolution: 1.932 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.41: pullulanase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	W381 D409 R412 F420	W373 D401 R404 F412
BS02	GLC	A	W381 N471	W373 N463
BS03	GLC	A	W234 D350 E379 H469 D470	W226 D342 E371 H461 D462
BS04	GLC	A	Y236 D237 N525 Y527	Y228 D229 N517 Y519
BS05	GLC	A	R495 D546 S549 S553	R487 D538 S541 S545
BS06	GLC	A	S553 F627 G628 E630	S545 F619 G620 E622
BS07	GLC	A	F627 G629	F619 G621
BS08	CA	A	D216 Y217 E224 E245	D208 Y209 E216 E237

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0046872 metal ion binding
• GO:0051060 pullulanase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:6jfj, PDBe:6jfj, PDBj:6jfj
• PDBsum: 6jfj
• PubMed: 32355041
• UniProt: A0A0C5GWS2