Structure of PDB 6je8 Chain A

Receptor sequence

>6je8A (length=469) Species: 349741 (Akkermansia muciniphila ATCC BAA-835)

TAQYSIIPEPSRTELRQETAKTLQLLSDQEVPTLETDAYRLTVTPQGAHL
ASGGREGRIYGLATLRQLRDQLAGQPEGIPCGVITDKPRYPWRGLMVDPA
RHFIPAADLKKFVDMMAYYKFNRLHLHLTDNQGWRLPVPGYPKLKSVASR
REESFGDGIPHEGMYTKQELKELVAYCAARGIDVIPEIDMPGHNQALHAA
YPEFFCFPKPDMNVRTTAGNSKELVCPQKPEVWKFYASVFNELKDIFPSG
IVHLGGDEAPTELWEKCPLCREARTRAAMKDEQEQMKAFFAKTAALLAKN
GQTPQFWYEGNAGIYHPGETVYAWRQGQALQSIEKTKKAGLNLIMASSEY
CYLDFPQIQGQRNWGWMKTTTLQKCYDLDPAFGKPEKEAGHIRGVHAPVW
AERLPDLNHLLYRAYPRACAIAEAGWSPMGVRSWENFRRKLADHRQFILK
RFNYDMERTQGNEPAFRWE

3D structure

• PDB: 6je8 Crystallographic evidence for substrate-assisted catalysis of beta-N-acetylhexosaminidas from Akkermansia muciniphila.
• Chain: A
• Resolution: 1.798 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D278 E279
• Catalytic site (residue number reindexed from 1): D257 E258
• Enzyme Commision number: 3.2.1.52: beta-N-acetylhexosaminidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C227 C247 C288	C206 C226 C267

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0004563 beta-N-acetylhexosaminidase activity
• GO:0016231 beta-N-acetylglucosaminidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0032428 beta-N-acetylgalactosaminidase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000272 polysaccharide catabolic process
• GO:0005975 carbohydrate metabolic process
• GO:0030203 glycosaminoglycan metabolic process
• GO:0052781 chitobiose catabolic process

Cellular Component

• GO:0016020 membrane

External links

• PDB: RCSB:6je8, PDBe:6je8, PDBj:6je8
• PDBsum: 6je8
• PubMed: 30846208
• UniProt: B2UP57|H2018_AKKM8 Beta-hexosaminidase Amuc_2018 (Gene Name=Amuc_2018)