Structure of PDB 6je4 Chain A

Receptor sequence

>6je4A (length=1049) Species: 604162 (Neisseria meningitidis 8013)

SINYILGLAIGIASVGWAMVEIDEEENPIRLIDLGVRVFERAEVPKTGDS
LAMARRLARSVRRLTRRRAHRLLRTRRLLKREGVLQAANFDENGLIKSLP
NTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNELGALLKGVAG
NAHALQTGDFRTPAELALNKFEKESGHIRNQRSDYSHTFSRKDLQAELIL
LFEKQKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAE
PKAAKNTYTAERFIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSK
LTYAQARKLLGLEDTAFFKGLRYGKDNAEASTLMEMKAYHAISRALEKEG
LKDKKSPLNLSPELQDEIGTAFSLFKTDEDITGRLKDRIQPEILEALLKH
ISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYGDNTEEKIYLPPIPA
DEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETAREVGKSFKDRKEI
EKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKCLYS
GKEINLGRLNEKGYVEIDAALPFSRTWDDSFNNKVLVLGSENQNKGNQTP
YEYFNGKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLND
TRYVNRFLCQFVADRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAEN
DRHHALDAVVVACSTVAMQQKITRFVRYKEMNAHQKTHFPQPWEFFAQEV
MIRVFGKPDGKPEFEEADTLEKLRTLLAEKLSSRPEAVHEYVTPLFVSRA
PNRKMSGQGHMETVKSAKRLDEGVSVLRVPLTQLKLKDLEKMVNREREPK
LYEALKARLEAHKDDPAKAFAEPFYKYDKAGNRTQQVKAVRVEQVQKTGV
WVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKGILPDRAVVQGK
DEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNINIR
IHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGKEIRPCRLKKRPPVR

3D structure

• PDB: 6je4 Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.
• Chain: A
• Resolution: 3.069 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Y140 R144 K145 G156 L158 L159 Y199 M254 L255 K266 N285 R287 K333 G334 K390 D418 K419 F420 R440 D442 R473 K656 F657 D658 Y672 R675 E845 T846 Q981 N1029 G1049 G1051 K1053 T1054	Y133 R137 K138 G142 L144 L145 Y185 M240 L241 K252 N271 R273 K319 G320 K376 D404 K405 F406 R426 D428 R454 K637 F638 D639 Y653 R656 E812 T813 Q948 N996 G1016 G1018 K1020 T1021
BS02	dna	A	P52 K53 V928 N944 T946 M947 Y965 H1024 R1033	P45 K46 V895 N911 T913 M914 Y932 H991 R1000
BS03	rna	A	S57 L58 A59 R62 R63 R66 R69 R70 L71 R73 R74 H77 R78 R81 R83 R84 R88 N108 P110 W111 H133 K136 R138 G139 G190 H191 I192 R193 N194 Q195 R196 Q242 R243 A245 T259 V421 Q422 Y441 P466 R473 R479 R485 K486 R493 R665 T670 R675 P834 R836 K837 M838 S839 G840 Q841 G842 H843 V847 S849 K851 R861 N877 R880 E881 K883 D911 T917 Q919 K921 A922 R924 R936 N939 S966 W967 A970 K971 E1065 R1071 C1073 P1080 V1081	S50 L51 A52 R55 R56 R59 R62 R63 L64 R66 R67 H70 R71 R74 R76 R77 R81 N101 P103 W104 H126 K129 R131 G132 G176 H177 I178 R179 N180 Q181 R182 Q228 R229 A231 T245 V407 Q408 Y427 P447 R454 R460 R466 K467 R474 R646 T651 R656 P801 R803 K804 M805 S806 G807 Q808 G809 H810 V814 S816 K818 R828 N844 R847 E848 K850 D878 T884 Q886 K888 A889 R891 R903 N906 S933 W934 A937 K938 E1032 R1038 C1040 P1047 V1048

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6je4, PDBe:6je4, PDBj:6je4
• PDBsum: 6je4
• PubMed: 31668930
• UniProt: C9X1G5|CAS9_NEIM8 CRISPR-associated endonuclease Cas9 (Gene Name=cas9)