Structure of PDB 6j3x Chain A

Receptor sequence

>6j3xA (length=415) Species: 304 (Roseateles saccharophilus)

DQAGKSPAGVRYHGGDEIILQGFHWNVVREAPNDWYNILRQQASTIAADG
FSAIWMPVPWRDFSSWTKSGGGEGYFWHDFNKNGRYGSDAQLRQAAGALG
GAGVKVLYDVVPNHMNRGYPDKEINLPAGQGFWRNDCADPGNYPNDCDDG
DRFIGGESDLNTGHPQIYGMFRDELANLRSGYGAGGFRFDFVRGYAPERV
NSWMTDSADSSFCVGELWKGPSEYPSWDWRNTASWQQIIKDWSDRAKCPV
FDFALKERMQNGSVADWKHGLNGNPDPRWREVAVTFVDNHDTGYSPGQNG
GQHHWALQDGLIRQAYAYILTSPGTPVVYWSHMYDWGYGDFIRQLIQVRR
TAGVRADSAISFHSGYSGLVATVSGSQQTLVVALNSDLANPGQVASGSFS
EAVNASNGQVRVWRS

3D structure

• PDB: 6j3x Maltotetraose-forming amylase from Pseudomonas saccharophila STB07
• Chain: A
• Resolution: 1.62 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R191 D193 E219 H293 D294
• Catalytic site (residue number reindexed from 1): R188 D190 E216 H290 D291
• Enzyme Commision number: 3.2.1.60: glucan 1,4-alpha-maltotetraohydrolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	W25 F156 Q305	W25 F153 Q302
BS02	GLC	A	W66 F79	W66 F76
BS03	GLC	A	I157 G158 E160	I154 G155 E157
BS04	CA	A	D1 Q2 H13 D16 E17	D1 Q2 H13 D16 E17
BS05	CA	A	N116 D151 D154 D162 G197	N113 D148 D151 D159 G194

Gene Ontology

Molecular Function

• GO:0004556 alpha-amylase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0030246 carbohydrate binding
• GO:0033910 glucan 1,4-alpha-maltotetraohydrolase activity
• GO:0043169 cation binding
• GO:0046872 metal ion binding
• GO:2001070 starch binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005983 starch catabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:6j3x, PDBe:6j3x, PDBj:6j3x
• PDBsum: 6j3x
• UniProt: P22963|AMT4_ROSSA Glucan 1,4-alpha-maltotetraohydrolase (Gene Name=mta)