Structure of PDB 6i8a Chain A

Receptor sequence

>6i8aA (length=1107) Species: 559292 (Saccharomyces cerevisiae S288C)

ALSAQQLLNASKIDDIDSMMGFERYVPPQYNGRFDAKDIDQIPGRVGWLT
NMHATLVSQENQGISGVDFYFLDEEGGSFKSTVVYDPYFFIACNDESRVN
DVEELVKKYLESCLKSLQIIRKEDLTMDNHLLGLQKTLIKLSFVNSNQLF
EARKLLRPILQDNANNNVQRNIYNVDAKHLIEDIREYDVPYHVRVSIDKD
IRVGKWYKVTQQGFIEDTRKIAFADPVVMAFDIETTKPPLKFRDSAVDQI
MMISYMIDGEGFLITNREIISEDIEDFEYTPKPEYPGFFTIFNENDEVAL
LQRFFEHIRDVRPTVISTFNGDFFDWPFIHNRSKIHGLDMFDEIGFAPDA
EGEYKSSYCSHMDCFRWVKRDSYLPQGSQGLKAVTQSKLGYNPIELDPEL
MTPYAFEKPQHLSEYSVSDAVATYYLYMKYVHPFIFSLCTIIPLNPDETL
RKGTGTLCEMLLMVQAYQHNILLPNKHTDPIERFYDGHLLESETYVGGHV
ESLEAGVFRSDLKNEFKIDPSAIDELLQELPEALKFSVEVENKSSVDKVT
NFEEIKNQITQKLLELKENNIRNELPLIYHVDVASMYPNIMTTNRLQPDS
IKAETCARKLKWAWRGEFFPSKMDEYNMIKRALQNETFPNKNLTFDELSY
ADQVIHIKKRLTEYSRKVYHRVKVSEIVEREAIVCQRENPFYVDTVKSFR
DRRYEFKGLAKTWKGNLSKIDPSDKHARDEAKKMIVLYDSLQLAHKVILN
SFYGYVMRKGSRWYSMEMAGITCLTGATIIQMARALVERVGRPLELDTDG
IWCILPKSFPETYFFTLENGKKLYLSYPCSMLNYRVHQKFTNHQYQELKD
PLNYIYETHSENTIFFEVDGPYKAMILPSSKEEGKGIKKRYAVFNEDGSL
AELKGFELKRRGELQLIKNFQSDIFKVFLEGDTLEGCYSAVASVCNRWLD
VLDSHGLMLEDEDLVSLICENRSMSKTLKEYEGQKSTSITTARRLGDFLG
EDMVKDKGLQCKYIISSKPFNAPVTERAIPVAIFSADIPIKRSFLRRWTL
DPSLEDLDIRTIIDWGYYRERLGSAIQKIITIPAALQGVSNPVPRVEHPD
WLKRKIA

3D structure

• PDB: 6i8a Structural consequence of the most frequently recurring cancer-associated substitution in DNA polymerase epsilon.
• Chain: A
• Resolution: 2.652 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P433 Q434 D875 T876 K967 Y969 K982 G983 K987 R988 R989 S1051 M1052 S1053 Y1059 Q1062	P375 Q376 D797 T798 K889 Y891 K904 G905 K909 R910 R911 S973 M974 S975 Y981 Q984
BS02	dna	A	K510 G511 T512 G513 T514 T552 Y553 G555 G556 V558 R686 V825 S829 G832 R836 I965 K966 K967 R968 R988 T1103 Y1145 K1156	K452 G453 T454 G455 T456 T494 Y495 G497 G498 V500 R615 V747 S751 G754 R758 I887 K888 K889 R890 R910 T1025 Y1067 K1078
BS03	DTP	A	D640 S643 M644 Y645 R781 K824 N828 D877	D582 S585 M586 Y587 R703 K746 N750 D799
BS04	CA	A	D640 V641 D877	D582 V583 D799
BS05	CA	A	D290 E292 D477	D232 E234 D419
BS06	FE	A	C677 C763	C606 C685

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006281 DNA repair

Cellular Component

• GO:0008622 epsilon DNA polymerase complex

External links

• PDB: RCSB:6i8a, PDBe:6i8a, PDBj:6i8a
• PDBsum: 6i8a
• PubMed: 30670696
• UniProt: P21951|DPOE_YEAST DNA polymerase epsilon catalytic subunit A (Gene Name=POL2)