Structure of PDB 6i02 Chain A

Receptor sequence

>6i02A (length=511) Species: 9606 (Homo sapiens)

LKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDR
FEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLST
QWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRNDWTVPKGC
FMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGSV
SVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLVT
DLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAFF
AASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLVR
AYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPSS
FVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTGS
GTIYDLRHFMLGIAPNLARWDFHTTHINQLQLLSTIDESPVFKEFVKRWK
SYLKGSRAKHN

3D structure

• PDB: 6i02 Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase.
• Chain: A
• Resolution: 2.45 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.17: heparosan-N-sulfate-glucuronate 5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	IDR	A	Q202 W203	Q101 W102
BS02	GNS	A	Q202 R428 Y500 Y560 R563 N572 R575 F578	Q101 R322 Y394 Y454 R457 N466 R469 F472
BS03	IDR	A	Q202 Y210 M445 E499 Y500 N510 Y560 F578	Q101 Y109 M339 E393 Y394 N404 Y454 F472
BS04	GNS	A	Y180 R185 R187 Y210 Q213 Q216 M445 Y514	Y79 R84 R86 Y109 Q112 Q115 M339 Y408
BS05	IDR	A	Y180 R185	Y79 R84
BS06	CA	A	T238 E240 T268 N269 D392	T137 E139 T162 N163 D286

Gene Ontology

Molecular Function

• GO:0047464 heparosan-N-sulfate-glucuronate 5-epimerase activity

Biological Process

• GO:0015012 heparan sulfate proteoglycan biosynthetic process

External links

• PDB: RCSB:6i02, PDBe:6i02, PDBj:6i02
• PDBsum: 6i02
• PubMed: 30872481
• UniProt: O94923|GLCE_HUMAN D-glucuronyl C5-epimerase (Gene Name=GLCE)