Structure of PDB 6i01 Chain A

Receptor sequence

>6i01A (length=512) Species: 9606 (Homo sapiens)

LKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDR
FEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLST
QWGPQGYFYPIQIAQYGLSHYSKNLTEKPPHIEVYETAEDRDNDWTVPKG
CFMANVADKSRFTNVKQFIAPETSEGVSLQLGNTKDFIISFDLKFLTNGS
VSVVLETTEKNQLFTIHYVSNAQLIAFKERDIYYGIGPRTSWSTVTRDLV
TDLRKGVGLSNTKAVKPTKIMPKKVVRLIAKGKGFLDNITISTTAHMAAF
FAASDWLVRNQDEKGGWPIMVTRKLGEGFKSLEPGWYSAMAQGQAISTLV
RAYLLTKDHIFLNSALRATAPYKFLSEQHGVKAVFMNKHDWYEEYPTTPS
SFVLNGFMYSLIGLYDLKETAGEKLGKEARSLYERGMESLKAMLPLYDTG
SGTIYDLRHFMLGIAPNLARWDFHTTHINQLQLLSTIDESPVFKEFVKRW
KSYLKGSRAKHN

3D structure

• PDB: 6i01 Substrate binding mode and catalytic mechanism of human heparan sulfate d-glucuronyl C5 epimerase.
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.17: heparosan-N-sulfate-glucuronate 5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BDP	A	Q202 W203	Q101 W102
BS02	GNS	A	Q202 R428 Y500 Y560 R563 R575 F578	Q101 R323 Y395 Y455 R458 R470 F473
BS03	BDP	A	Q202 M445 E499 Y500 N510 Y560 F578	Q101 M340 E394 Y395 N405 Y455 F473
BS04	GNS	A	Y180 R185 R187 Y210 Q213 Q216 M445 Y514	Y79 R84 R86 Y109 Q112 Q115 M340 Y409
BS05	BDP	A	Y180 R185	Y79 R84
BS06	MAN	A	I233 N393	I132 N288
BS07	CA	A	T238 E240 T268 N269 D392	T137 E139 T163 N164 D287

Gene Ontology

Molecular Function

• GO:0047464 heparosan-N-sulfate-glucuronate 5-epimerase activity

Biological Process

• GO:0015012 heparan sulfate proteoglycan biosynthetic process

External links

• PDB: RCSB:6i01, PDBe:6i01, PDBj:6i01
• PDBsum: 6i01
• PubMed: 30872481
• UniProt: O94923|GLCE_HUMAN D-glucuronyl C5-epimerase (Gene Name=GLCE)