Structure of PDB 6hqn Chain A

Receptor sequence

>6hqnA (length=403) Species: 385957 (Amycolatopsis sp. ATCC 39116)

ERPDLAWLDEVTMTQLERNPYEVYERLRAEAPLAFVPVLGSYVASTAEVC
REVATSPDFEAVITPAGGRTFGHPAIIGVNGDIHADLRSMVEPALQPAEV
DRWIDDLVRPIARRYLERFENDGHAELVAQYCEPVSVRSLGDLLGLQEVD
SDKLREWFAKLNRSLTNAAVDENGEFANPEGFAEGDQAKAEIRAVVDPLI
DKWIEHPDDSAISHWLHDGMPPGQTRDREYIYPTIYVYLLGAMQEPGHGM
ASTLVGLFSRPEQLEEVVDDPTLIPRAIAEGLRWTSPIWSATARISTKPV
TIAGVDLPAGTPVMLSYGSANHDTGKYEAPSQYDLHRPPLPHLAFGAGNH
ACAGIYFANHVMRIALEELFEAIPNLERDTREGVEFWGWGFRGPTSLHVT
WEV

3D structure

• PDB: 6hqn Enabling microbial syringol conversion through structure-guided protein engineering.
• Chain: A
• Resolution: 1.87 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R167 Q248 E249 C356 A357 G358
• Catalytic site (residue number reindexed from 1): R163 Q244 E245 C352 A353 G354
• Enzyme Commision number: 1.14.14.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	A	I81 H88 R92 Y242 A246 E249 P250 I292 T296 R298 Y321 A348 F349 G350 H354 A355 C356 F361 A362 M366	I77 H84 R88 Y238 A242 E245 P246 I288 T292 R294 Y317 A344 F345 G346 H350 A351 C352 F357 A358 M362
BS02	JZ3	A	V241 L244 G245 A246 I292 T296	V237 L240 G241 A242 I288 T292

Gene Ontology

Molecular Function

• GO:0004497 monooxygenase activity
• GO:0005506 iron ion binding
• GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
• GO:0020037 heme binding
• GO:0046872 metal ion binding

Biological Process

• GO:0009056 catabolic process

External links

• PDB: RCSB:6hqn, PDBe:6hqn, PDBj:6hqn
• PDBsum: 6hqn
• PubMed: 31235604
• UniProt: P0DPQ7|GCOA_AMYS7 Aromatic O-demethylase, cytochrome P450 subunit (Gene Name=gcoA)