Structure of PDB 6haf Chain A

Receptor sequence

>6hafA (length=585) Species: 1590 (Lactiplantibacillus plantarum)

TNILAGAAVIKVLEAWGVDHLYGIPGGSINSIMDALSAERDRIHYIQVRH
QEVGAMAAAADAKLTGKIGVCFGSAGPGGTHLMNGLYDAREDHVPVLALI
GQFGTTGMNMDTFQEMNENPIYADVADYNVTAVNAATLPHVIDEAIRRAY
AHQGVAVVQIPVDLPWQQIPAEDWYASANSYQTPLLPEPDVQAVTRLTQT
LLAAERPLIYYGIGARKAGKELEQLSKTLKIPLMSTYPAKGIVADRYPAY
LGSANRVAQKPANEALAQADVVLFVGNNYPFAEVSKAFKNTRYFLQIDID
PAKLGKRHKTDIAVLADAQKTLAAILAQVSERESTPWWQANLANVKNWRA
YLASLEDKQEGPLQAYQVLRAVNKIAEPDAIYSIDVGDINLNANRHLKLT
PSNRHITSNLFATMGVGIPGAIAAKLNYPERQVFNLAGDGGASMTMQDLA
TQVQYHLPVINVVFTNCQYGFIKDEQEDTNQNDFIGVEFNDIDFSKIADG
VHMQAFRVNKIEQLPDVFEQAKAIAQHEPVLIDAVITGDRPLPAEKLRLD
SAMSSAADIEAFKQRYEAQDLQPLSTYLKQFGLDD

3D structure

• PDB: 6haf Low-barrier hydrogen bonds in enzyme cooperativity.
• Chain: A
• Resolution: 1.3 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): I32 G34 G35 S36 I37 Q59 S82 F121 Q122 E123 V170 R264 E291 V394 A420 M422 D447 N474 Q476 Y477 F479 I480 E483
• Catalytic site (residue number reindexed from 1): I24 G26 G27 S28 I29 Q51 S74 F113 Q114 E115 V162 R256 E283 V386 A412 M414 D439 N466 Q468 Y469 F471 I472 E475
• Enzyme Commision number: 1.2.3.3: pyruvate oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	H101 G220 I221 G222 T244 Y245 A262 N263 V265 G284 N285 N286 P288 F289 D306 I307 K311 D325 A326 N417	H93 G212 I213 G214 T236 Y237 A254 N255 V257 G276 N277 N278 P280 F281 D298 I299 K303 D317 A318 N409
BS02	MG	A	D447 N474 Q476	D439 N466 Q468
BS03	TPP	A	D396 M422 G446 G448 N474 Q476 Y477 G478 F479 I480	D388 M414 G438 G440 N466 Q468 Y469 G470 F471 I472

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0016491 oxidoreductase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0046872 metal ion binding
• GO:0047112 pyruvate oxidase activity

External links

• PDB: RCSB:6haf, PDBe:6haf, PDBj:6haf
• PDBsum: 6haf
• PubMed: 31534226
• UniProt: P37063|POXB_LACPL Pyruvate oxidase (Gene Name=pox5)