Structure of PDB 6h80 Chain A

Receptor sequence

>6h80A (length=558) Species: 408692 (Dengue virus 3 Sri Lanka/1266/2000)

NMDVIGERIKRIKEEHNSTWHYDDENPYKTWAYHGSYEVKMINGVVKLLT
KPWDVVPMVTQMAMKEKVDTRTPRPLPGTRKVMEITAEWLWRTLGRNKRP
RLCTREEFTKKVRTNDSAKAAVEDEEFWKLVDRERELHKLGKCGSCVYNM
SRAIWYMWLGVRYLEFEALGFLNEDHWFSRENSYSGVEGEGLHKLGYILR
DISKIPGGAMYADDTAGWDTRITEDDLHNEEKIIQQMDPEHRQLANAIFK
LTYQNKVVKVQTVMDIISRKDQRGSGQVGTYGLNTFTNMEAQLVRQMEGE
GVLTKADLENPHLLEKKITQWLETKGVERLKRMAISGDDCVVKPIDDRFA
NALLALNDMGKVRKDIPQWQPSKGWHDWQQVPFCSHHFHELIMKDGRKLV
VPCRPQDELIGRARISQGAGWSLRETACLGKAYAQMWSLMYFHRRDLRLA
SNAICSAVPVHWVPTSRTTWSIHAHHQWMTTEDMLTVWNRVWIEENPWME
DKTPVTTWENVPYLGKREDQWCGSLIGLTSRATWAQNIPTAIQQVRSLIG
NEEFLDYM

3D structure

• PDB: 6h80 Development and validation of RdRp Screen, a crystallization screen for viral RNA-dependent RNA polymerases.
• Chain: A
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  3.4.21.91: flavivirin.
  3.6.1.15: nucleoside-triphosphate phosphatase.
  3.6.4.13: RNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	5V5	A	L511 C709 H711 R729 T794 S796 H798 A799 W803	L192 C384 H386 R404 T469 S471 H473 A474 W478
BS02	ZN	A	C446 C449	C143 C146
BS03	ZN	A	H712 H714 C728 C847	H387 H389 C403 C522

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0005524 ATP binding

Biological Process

• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:6h80, PDBe:6h80, PDBj:6h80
• PDBsum: 6h80
• PubMed: 30602529
• UniProt: Q6YMS4|POLG_DEN3S Genome polyprotein (Gene Name=pol)