Structure of PDB 6gtg Chain A

Receptor sequence

>6gtgA (length=1298) Species: 401614 (Francisella tularensis subsp. novicida U112)

IYQEFVNKYSLSKTLRFELIPQGKTLENIKARGLILDDEKRAKDYKKAKQ
IIDKYHQFFIEEILSSVCISEDLLQNYSDVYFKLKKSDDDNLQKDFKSAK
DTIKKQISEYIKDSEKFKNLFNQNLIDAKKGQESDLILWLKQSKDNGIEL
FKANSDITDIDEALEIIKSFKGWTTYFKGFHENRKNVYSSNDIPTSIIYR
IVDDNLPKFLENKAKYESLKDKAPEAINYEQIKKDLAEELTFDIDYKTSE
VNQRVFSLDEVFEIANFNNYLNQSGITKFNTIIGGKFVNGENTKRKGINE
YINLYSQQINDKTLKKYKMSVLFKQILSDTESKSFVIDKLEDDSDVVTTM
QSFYEQIAAFKTVEEKSIKETLSLLFDDLKAQKLDLSKIYFKNDKSLTDL
SQQVFDDYSVIGTAVLEYITQQIAPKNLDNPSKKEQELIAKKTEKAKYLS
LETIKLALEEFNKHRDIDKQCRFEEILANFAAIPMIFDEIAQNKDNLAQI
SIKYQNQGKKDLLQASAEDDVKAIKDLLDQTNNLLHKLKIFHISQSEDKA
NILDKDEHFYLVFEECYFELANIVPLYNKIRNYITQKPYSDEKFKLNFEN
STLANGWDKNKEPDNTAILFIKDDKYYLGVMNKKNNKIFDDKAIKENKGE
GYKKIVYKLLPGANKMLPKVFFSAKSIKFYNPSEDILRIRNHSTHTKNGS
PQKGYEKFEFNIEDCRKFIDFYKQSISKHPEWKDFGFRFSDTQRYNSIDE
FYREVENQGYKLTFENISESYIDSVVNQGKLYLFQIYNKDFSAYSKGRPN
LHTLYWKALFDERNLQDVVYKLNGEAELFYRKQSIPKKITHPAKEAIANK
NKDNPKKESVFEYDLIKDKRFTEDKFFFHCPITINFKSSGANKFNDEINL
LLKEKANDVHILSIDRGERHLAYYTLVDGKGNIIKQDTFNIIGNDRMKTN
YHDKLAAIEKDRDSARKDWKKINNIKEMKEGYLSQVVHEIAKLVIEYNAI
VVFQDLNFGFKRGRFKVEKQVYQKLEKMLIEKLNYLVFKDNEFDKTGGVL
RAYQLTAPFETFKKMGKQTGIIYYVPAGFTSKICPVTGFVNQLYPKYESV
SKSQEFFSKFDKICYNLDKGYFEFSFDYKNFGDKAAKGKWTIASFGSRLI
NFRNSDKNHNWDTREVYPTKELEKLLKDYSIEYGHGECIKAAICGESDKK
FFAKLTSVLNTILQMRNSKTGTELDYLISPVADVNGNFFDSRQAPKNMPQ
DADANGAYHIGLKGLMLLGRIKNNQEGKKLNLVIKNEEYFEFVQNRNN

3D structure

• PDB: 6gtg Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
• Chain: A
• Resolution: 3.27 Å

Enzymatic activity

• Enzyme Commision number: 3.1.21.1: deoxyribonuclease I.
  4.6.1.22: Bacillus subtilis ribonuclease.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S14 K15 T16 R18 K51 N185 R186 R202 F289 T295 K296 L306 Q309 F325 K326 I328 L329 K447 A448 H538 Y579 N580 R583 N790 K791 Y796 S797 K798 R800 L803 H804 R833 H843 I849 A850 N851 K852 N856 K858 S861 F863 Y865 L867 K869 K871 R872 F873 F879 H881 R948 M949 Y953 K956 R968 K969 E979 M980 G983 S986 H990 R1014 F1017 K1018 K1034 K1041 N1160	S12 K13 T14 R16 K49 N183 R184 R200 F287 T293 K294 L304 Q307 F323 K324 I326 L327 K445 A446 H536 Y577 N578 R581 N788 K789 Y794 S795 K796 R798 L801 H802 R831 H841 I847 A848 N849 K850 N854 K856 S859 F861 Y863 L865 K867 K869 R870 F871 F877 H879 R946 M947 Y951 K954 R966 K967 E977 M978 G981 S984 H988 R1012 F1015 K1016 K1032 K1039 N1158
BS02	dna	A	K131 K132 N188 I195 P196 K296 N301 E302 N305 K317 K320 V338 K341 K397 T400 D401 Q404 Y410 N584 Q588 K589 G608 D610 N612 K613 L661 P663 M668 K671 V672 S675 A676 K677 W734 K823 N825 E827 R964 N976 I977 K978 K1026 T1063 F1064 K1065 K1159 H1161	K129 K130 N186 I193 P194 K294 N299 E300 N303 K315 K318 V336 K339 K395 T398 D399 Q402 Y408 N582 Q586 K587 G606 D608 N610 K611 L659 P661 M666 K669 V670 S673 A674 K675 W732 K821 N823 E825 R962 N974 I975 K976 K1024 T1061 F1062 K1063 K1157 H1159
BS03	dna	A	N124 Q125 G174 T177 N666 K667 K671 T698 K699 D751 K895	N122 Q123 G172 T175 N664 K665 K669 T696 K697 D749 K893
BS04	MG	A	G799 R800 P801	G797 R798 P799

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004530 deoxyribonuclease I activity
• GO:0016829 lyase activity

Biological Process

• GO:0006259 DNA metabolic process
• GO:0051607 defense response to virus

External links

• PDB: RCSB:6gtg, PDBe:6gtg, PDBj:6gtg
• PDBsum: 6gtg
• PubMed: 30503205
• UniProt: A0Q7Q2|CS12A_FRATN CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)