Structure of PDB 6go7 Chain A

Receptor sequence

>6go7A (length=338) Species: 10090 (Mus musculus)

KISQYACQRRTTLNNYNQLFTDALDILAENDELRENEGSCLAFMRASSVL
KSLPFPITSMKDTEGIPCLGDKVKSIIEGIIEDGESSEAKAVLNDERYKS
FKLFTSVFGVGLKTAEKWFRMGFRTLSKIQSDKSLRFTQMQKAGFLYYED
LVSCVNRPEAEAVSMLVKEAVVTFLPDALVTMTGGFRRGKMTGHDVDFLI
TSPEATEDEEQQLLHKVTDFWKQQGLLLYHQYHRSFERSFCILKLDHGRV
HSGKGWKAIRVDLVMCPYDRRAFALLGWTGSRQFERDLRRYATHERKMML
DNHALYDRTKRVFLEAESEEEIFAHLGLDYIEPWERNA

3D structure

• PDB: 6go7 Structural evidence for an intransbase selection mechanism involving Loop1 in polymerase mu at an NHEJ double-strand break junction.
• Chain: A
• Resolution: 2.55 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.31: DNA nucleotidylexotransferase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q152 G186 S187 A190 G218 K220 V221 H467	Q4 G38 S39 A42 G70 K72 V73 H294
BS02	dna	A	G257 G259 K261 T262 H342 H381 R404 F406	G109 G111 K113 T114 H194 H233 R238 F240
BS03	dna	A	L189 R455 R459 R462 T466 H467	L41 R282 R286 R289 T293 H294
BS04	dna	A	K265 T286 Q287 H381 R382	K117 T138 Q139 H233 R234
BS05	DCT	A	G332 G333 G341 H342 D343 D345 W451 G453 R455	G184 G185 G193 H194 D195 D197 W278 G280 R282
BS06	MG	A	D343 D345	D195 D197

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:6go7, PDBe:6go7, PDBj:6go7
• PDBsum: 6go7
• PubMed: 31138645
• UniProt: P09838|TDT_MOUSE DNA nucleotidylexotransferase (Gene Name=Dntt);
  Q9JIW4|DPOLM_MOUSE DNA-directed DNA/RNA polymerase mu (Gene Name=Polm)