Structure of PDB 6f94 Chain A

Receptor sequence
>6f94A (length=187) Species: 83334 (Escherichia coli O157:H7) [Search protein sequence]
GLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIH
ADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLGVGVSVVNALSQKLEL
VIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVTEF
EYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYE
3D structure
PDB6f94 New insights into the binding mode of pyridine-3-carboxamide inhibitors of E. coli DNA gyrase.
ChainA
Resolution2.35 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 D0H A V43 A47 E50 D73 R76 G77 I78 I94 R136 V29 A33 E36 D59 R62 G63 I64 I80 R104 MOAD: ic50=0.099uM
PDBbind-CN: -logKd/Ki=7.00,IC50=99nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524 ATP binding
Biological Process
GO:0006265 DNA topological change

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Molecular Function
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Biological Process
External links
PDB RCSB:6f94, PDBe:6f94, PDBj:6f94
PDBsum6f94
PubMed31257079
UniProtP0AES6|GYRB_ECOLI DNA gyrase subunit B (Gene Name=gyrB)

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