Structure of PDB 6f8b Chain A

Receptor sequence
>6f8bA (length=298) Species: 287 (Pseudomonas aeruginosa) [Search protein sequence]
AEAGGPGGNQKIGKYTYGSDYGPLIVNDRCEMDDGNVITVDMNSSTDDSK
TTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFKLYRDWFGTSPLTHKL
YMKVHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQN
SGLIYRGQSGGMNEAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYM
DQPSRDGRSIDNASQYYNGIDVHHSSGVYNRAFYLLANSPGWDTRKAFEV
FVDANRYYWTATSNYNSGACGVIRSAQNRNYSAADVTRAFSTVGVTCP
3D structure
PDB6f8b Binding Mode Characterization and Early in Vivo Evaluation of Fragment-Like Thiols as Inhibitors of the Virulence Factor LasB from Pseudomonas aeruginosa.
ChainA
Resolution1.3 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) H140 E141 H144 Y155 E164 D221 H223
Catalytic site (residue number reindexed from 1) H140 E141 H144 Y155 E164 D221 H223
Enzyme Commision number 3.4.24.26: pseudolysin.
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 CA A D136 E172 E175 D183 L185 D136 E172 E175 D183 L185
BS02 ZN A H140 H144 E164 H140 H144 E164
BS03 CXH A H140 E141 R198 H223 H140 E141 R198 H223 MOAD: ic50=6.6uM
PDBbind-CN: -logKd/Ki=5.21,IC50=6.2uM
BindingDB: IC50=6600nM
BS04 CXH A N112 V137 H140 E141 L197 N112 V137 H140 E141 L197 MOAD: ic50=6.6uM
PDBbind-CN: -logKd/Ki=5.21,IC50=6.2uM
BindingDB: IC50=6600nM
Gene Ontology
Molecular Function
GO:0004222 metalloendopeptidase activity
Biological Process
GO:0006508 proteolysis

View graph for
Molecular Function

View graph for
Biological Process
External links
PDB RCSB:6f8b, PDBe:6f8b, PDBj:6f8b
PDBsum6f8b
PubMed29485268
UniProtP14756|ELAS_PSEAE Elastase (Gene Name=lasB)

[Back to BioLiP]