Structure of PDB 6etj Chain A

Receptor sequence

>6etjA (length=432) Species: 9606 (Homo sapiens) [Search protein sequence]

ELTQSRVQKIWVPVDHRPSLSPTVIVMVGLPARGKTYISKKLTRYLNWIG
VPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSY
LAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVAS
NIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLI
KVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQG
RIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRL
PYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESY
QDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCP
LHTVLKLTPVAYGCRVESIYLNVESVCTHRER

3D structure

PDB

6etj Targeting PFKFB3 radiosensitizes cancer cells and suppresses homologous recombination.

Chain

Resolution

2.51 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R252 H253 N259 S302 E322 H387
Catalytic site (residue number reindexed from 1)	R240 H241 N247 S290 E310 H375
Enzyme Commision number	2.7.1.105: 6-phosphofructo-2-kinase. 3.1.3.46: fructose-2,6-bisphosphate 2-phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F6P	A	H253 I264 G265 E322 Y333 R347 K351 Y362 Q388 R392	H241 I252 G253 E310 Y321 R335 K339 Y350 Q376 R380
BS02	BWW	A	V70 G71 R74 F87 M95 R98 A125 T126 N127 R132 Y193	V58 G59 R62 F75 M83 R86 A113 T114 N115 R120 Y181	PDBbind-CN: -logKd/Ki=6.72,IC50=0.19uM
BS03	ADP	A	A44 R45 G46 K47 T48 Y49 N163 E166 V167 K168 V217 Y424	A32 R33 G34 K35 T36 Y37 N151 E154 V155 K156 V205 Y412

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0003873	6-phosphofructo-2-kinase activity
GO:0004331	fructose-2,6-bisphosphate 2-phosphatase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016787	hydrolase activity

	Biological Process
GO:0006000	fructose metabolic process
GO:0006003	fructose 2,6-bisphosphate metabolic process
GO:0006096	glycolytic process
GO:0006915	apoptotic process
GO:0010001	glial cell differentiation
GO:0016310	phosphorylation
GO:0046835	carbohydrate phosphorylation
GO:0061744	motor behavior

	Cellular Component
GO:0005654	nucleoplasm
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:6etj, PDBe:6etj, PDBj:6etj
PDBsum	6etj
PubMed	30250201
UniProt	Q16875\|F263_HUMAN 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (Gene Name=PFKFB3)

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