Structure of PDB 6ech Chain A

Receptor sequence

>6echA (length=528) Species: 10116 (Rattus norvegicus) [Search protein sequence]

QELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPAS
RSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSY
RPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAK
TVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGS
RKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVR
DALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEK
VFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDG
ADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLS
RDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTR
SAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRV
GDLVIVVTGWRPGSGYTNIMRVLSVSHH

3D structure

PDB

6ech Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production.

Chain

Resolution

2.19 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R85 R132 K282 T340
Catalytic site (residue number reindexed from 1)	R68 R115 K265 T323
Enzyme Commision number	2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	FBP	A	L443 T444 K445 T446 S449 R501 G526 R528 P529 G530 S531 Y533 T534	L426 T427 K428 T429 S432 R484 G509 R511 P512 G513 S514 Y516 T517
BS02	MG	A	E284 D308	E267 D291
BS03	MG	A	N87 S89 D125 T126	N70 S72 D108 T109
BS04	ATP	A	P65 R85 N87 H90 R132 K219	P48 R68 N70 H73 R115 K202
BS05	OXL	A	K282 E284 A305 G307 D308 T340	K265 E267 A288 G290 D291 T323

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0004743	pyruvate kinase activity
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0030955	potassium ion binding
GO:0046872	metal ion binding
GO:0048029	monosaccharide binding

	Biological Process
GO:0001666	response to hypoxia
GO:0006096	glycolytic process
GO:0007584	response to nutrient
GO:0009749	response to glucose
GO:0010038	response to metal ion
GO:0016310	phosphorylation
GO:0032869	cellular response to insulin stimulus
GO:0033198	response to ATP
GO:0042866	pyruvate biosynthetic process
GO:0051591	response to cAMP
GO:0051707	response to other organism
GO:0071872	cellular response to epinephrine stimulus

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005886	plasma membrane

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:6ech, PDBe:6ech, PDBj:6ech
PDBsum	6ech
PubMed	31825824
UniProt	P12928\|KPYR_RAT Pyruvate kinase PKLR (Gene Name=Pklr)

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