Structure of PDB 6dcb Chain A

Receptor sequence

>6dcbA (length=225) Species: 9606 (Homo sapiens)

RKFQYGNYCKYYGYRNPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLS
IACKWGPSRMVGLDIDSRLIHSARQNIRHYLSTSVFPNNVVFVTGNYVLD
RDDLVEAQTPEYDVVLCLSLTKWVHLNWGDEGLKRMFRRIYRHLRPGGIL
VLEPQPWSSYGKRKTLTETIYKNYYRIQLKPEQFSSYLTSPDVGFSSYEL
VATPHNTSKGFQRPVYLFHKARSPS

3D structure

• PDB: 6dcb Structural basis of 7SK RNA 5'-gamma-phosphate methylation and retention by MePCE.
• Chain: A
• Resolution: 1.998 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y418 C419 K420 Y421 G423 Y424 H456 S482 Q485 N486 R488 H489 Y490 K585 W586 Q618 R626 S671 G673 F674 R676	Y8 C9 K10 Y11 G13 Y14 H46 S72 Q75 N76 R78 H79 Y80 K122 W123 Q155 R163 S208 G210 F211 R213	PDBbind-CN: Kd=100nM
BS02	SAH	A	Y415 G416 N417 Y421 Y422 R433 G451 N453 D474 I475 N559 L581 S582 L583 W591	Y5 G6 N7 Y11 Y12 R23 G41 N43 D64 I65 N96 L118 S119 L120 W128

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity

External links

• PDB: RCSB:6dcb, PDBe:6dcb, PDBj:6dcb
• PDBsum: 6dcb
• PubMed: 30559425
• UniProt: Q7L2J0|MEPCE_HUMAN 7SK snRNA methylphosphate capping enzyme (Gene Name=MEPCE)