Structure of PDB 6czo Chain A

Receptor sequence

>6czoA (length=293) Species: 9606 (Homo sapiens)

SLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLEL
EAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETIC
LLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDC
FNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLL
WSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGY
EFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKP

3D structure

• PDB: 6czo KNL1 Binding to PP1 and Microtubules Is Mutually Exclusive.
• Chain: A
• Resolution: 2.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D64 H66 D92 D95 R96 N124 H125 H173 R221 H248
• Catalytic site (residue number reindexed from 1): D59 H61 D87 D90 R91 N119 H120 H168 R216 H243
• Enzyme Commision number: 3.1.3.16: protein-serine/threonine phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	E54 L55 E56 R74 Y78 N86 E116 N117 F119 D166 D242 L243 F257 L289 M290 C291 F293 Q294 I295 K297 P298	E49 L50 E51 R69 Y73 N81 E111 N112 F114 D161 D237 L238 F252 L284 M285 C286 F288 Q289 I290 K292 P293
BS02	peptide	A	E77 Y255 R261	E72 Y250 R256
BS03	MN	A	D64 H66 D92	D59 H61 D87
BS04	MN	A	D92 N124 H173 H248	D87 N119 H168 H243

Gene Ontology

Molecular Function

• GO:0016787 hydrolase activity

External links

• PDB: RCSB:6czo, PDBe:6czo, PDBj:6czo
• PDBsum: 6czo
• PubMed: 30100357
• UniProt: P62136|PP1A_HUMAN Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (Gene Name=PPP1CA)