Structure of PDB 6cyv Chain A

Receptor sequence
>6cyvA (length=155) Species: 199310 (Escherichia coli CFT073) [Search protein sequence]
MISLIAALAVDRVIPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPG
RKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPK
AQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEIL
ERRHH
3D structure
PDB6cyv The crystal structure of a tetrahydrofolate-bound dihydrofolate reductase reveals the origin of slow product release.
ChainA
Resolution1.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) I5 W22 D27 L28 F31 L54 I91 T113
Catalytic site (residue number reindexed from 1) I5 W16 D21 L22 F25 L48 I85 T107
Enzyme Commision number 1.5.1.3: dihydrofolate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAP A G43 R44 H45 T46 L62 S63 S64 K76 G96 G97 R98 V99 Q102 G37 R38 H39 T40 L56 S57 S58 K70 G90 G91 R92 V93 Q96
BS02 DHF A A6 A7 D27 F31 I50 R57 A6 A7 D21 F25 I44 R51
Gene Ontology
Molecular Function
GO:0004146 dihydrofolate reductase activity
GO:0005515 protein binding
GO:0005542 folic acid binding
GO:0016491 oxidoreductase activity
GO:0050661 NADP binding
GO:0051870 methotrexate binding
GO:0051871 dihydrofolic acid binding
GO:0070401 NADP+ binding
GO:0070402 NADPH binding
Biological Process
GO:0006730 one-carbon metabolic process
GO:0009257 10-formyltetrahydrofolate biosynthetic process
GO:0009410 response to xenobiotic stimulus
GO:0031427 response to methotrexate
GO:0046452 dihydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046655 folic acid metabolic process
GO:0046656 folic acid biosynthetic process
GO:0046677 response to antibiotic
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6cyv, PDBe:6cyv, PDBj:6cyv
PDBsum6cyv
PubMed30564747
UniProtP0ABQ4|DYR_ECOLI Dihydrofolate reductase (Gene Name=folA)

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