Structure of PDB 6cxx Chain A

Receptor sequence

>6cxxA (length=374) Species: 9796 (Equus caballus)

STAGKVIKCKAAVLWEEKKPFSIEEVEVAPPKAHEVRIKMVATGICRSDD
HVVSGTLVTPLPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKC
RVCKHPEGNFCLKNDLSMPRGTMQDGTSRFTCRGKPIHHFLGTSTFSQYT
VVDEISVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTQGSTCAVFGL
GGVGLSVIMGCKAAGAARIIGVDINKDKFAKAKEVGATECVNPQDYKKPI
QEVLTEMSNGGVDFSFHVIGRLDTMVTALSCCQEAYGVSVIVGVPPDSQN
LSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVL
PFEKINEGFDLLRSGESIRTILTF

3D structure

• PDB: 6cxx Substitutions of a buried glutamate residue hinder the conformational change in horse liver alcohol dehydrogenase and yield a surprising complex with endogenous 3'-Dephosphocoenzyme A.
• Chain: A
• Resolution: 1.26 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C46 S48 H51 H67 C174
• Catalytic site (residue number reindexed from 1): C46 S48 H51 H67 C174
• Enzyme Commision number: 1.1.1.1: alcohol dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C46 H67 C174	C46 H67 C174
BS02	ZN	A	C97 C100 C103 C111	C97 C100 C103 C111
BS03	COD	A	R47 S48 F93 C174 G201 V203 D223 I224 I269 R271 T274 G293 V294	R47 S48 F93 C174 G201 V203 D223 I224 I269 R271 T274 G293 V294

Gene Ontology

Molecular Function

• GO:0004022 alcohol dehydrogenase (NAD+) activity
• GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042572 retinol metabolic process
• GO:0042573 retinoic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:6cxx, PDBe:6cxx, PDBj:6cxx
• PDBsum: 6cxx
• PubMed: 30018019
• UniProt: P00327|ADH1E_HORSE Alcohol dehydrogenase E chain