Structure of PDB 6crm Chain A

Receptor sequence

>6crmA (length=512) Species: 290339 (Cronobacter sakazakii ATCC BAA-894)

EVYSQETLAKQVLQETFGYQQFRPGQATIIDAVLEGRDCLVVMPTGGGKS
LCYQIPALVKTGLTIVVSPLISLMKDQVDQLLANGVAAACLNSTQSREEQ
QAVLAGCRTGQVRLLYIAPERLMMDNFIDTLGYWDLAMVAVDEAHCISQW
GHDFRPEYAALGQLRARFPAVPFMALTATADDTTRRDIVRLLGLDDPLIE
ISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAA
RLQSRGISAAAYHAGLEHEVRASVQEKFQRDDLQIVVATVAFGMGINKPN
VRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEE
KAPGPLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQAPCGNCDICL
DPPRRYDGLVDAQKALSAIARVEQRFGMGYVVEVLRGANNQRIRELGHDK
LKVYGIGRDQSQEHWVSVIRQLIHLGVVTQNIAQHSALQLTEAARPFLRG
EAPLMLAVPRVA

3D structure

• PDB: 6crm A guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases.
• Chain: A
• Resolution: 2.19286 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L74 I75 S97 E124 R125 W154 F158 R159 K222 P223 N244 S245 R246 K248 H267 A268 R275 T293 D312 R315 D344 W347 C351 K355	L70 I71 S93 E120 R121 W150 F154 R155 K218 P219 N240 S241 R242 K244 H263 A264 R271 T289 D308 R311 D340 W343 C347 K351	PDBbind-CN: Kd=4.7uM
BS02	ZN	A	C380 C397 C400 C403	C376 C393 C396 C399

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0043138 3'-5' DNA helicase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0009432 SOS response

External links

• PDB: RCSB:6crm, PDBe:6crm, PDBj:6crm
• PDBsum: 6crm
• PubMed: 30305632
• UniProt: A7MQK9