Structure of PDB 6c71 Chain A

Receptor sequence

>6c71A (length=432) Species: 1042876 (Pseudomonas putida S16) [Search protein sequence]

GFDYDVVVVGGGFAGATAARECGLQGYRTLLLEARSRLGGRTFTSRFAGQ
EIEFGGAWVHWLQPHVWAEMQRYGLGVVEDPLTNLDKTLIMYNDGSVESI
SPDEFGKNIRIAFEKLCHDAWEVFPRPHEPMFTERARELDKSSVLDRIKT
LGLSRLQQAQINSYMALYAGETTDKFGLPGVLKLFACGGWNYDAFMDTET
HYRIQGGTIGLINAMLTDSGAEVRMSVPVTAVEQVNGGVKIKTDDDEIIT
AGVVVMTVPLNTYKHIGFTPALSKGKQRFIKEGQLSKGAKLYVHVKQNLG
RVFAFADEQQPLNWVQTHDYSDELGTILSITIARKETIDVNDRDAVTREV
QKMFPGVEVLGTAAYDWTADPFSLGAWAAYGVGQLSRLKDLQAAEGRILF
AGAETSNGWHANIDGAVESGLRAGREVKQLLS

3D structure

PDB

6c71 Crystallography Coupled with Kinetic Analysis Provides Mechanistic Underpinnings of a Nicotine-Degrading Enzyme.

Chain

Resolution

2.649 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	H110
Catalytic site (residue number reindexed from 1)	H60
Enzyme Commision number	1.4.2.2: nicotine dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NCT	A	L217 Y218 T250 W364 T381 W427 A461	L167 Y168 T200 W314 T331 W377 A411
BS02	FAD	A	G60 G62 A64 E83 A84 R85 G90 R91 T92 G105 G106 A107 W108 P278 V279 V308 P309 I316 W417 W427 G452 A453 N462 I463 A466	G10 G12 A14 E33 A34 R35 G40 R41 T42 G55 G56 A57 W58 P228 V229 V258 P259 I266 W367 W377 G402 A403 N412 I413 A416

Gene Ontology

	Molecular Function
GO:0016491	oxidoreductase activity

View graph for
Molecular Function

External links

PDB	RCSB:6c71, PDBe:6c71, PDBj:6c71
PDBsum	6c71
PubMed	29812904
UniProt	F8G0P2\|NICA2_PSEP6 Nicotine dehydrogenase (Gene Name=nicA2)

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