Structure of PDB 6c0v Chain A

Receptor sequence

>6c0vA (length=1154) Species: 9606 (Homo sapiens)

SVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFAMNLE
EDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQ
EIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGF
TRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVL
AAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYA
SYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANA
RGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVK
ILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDI
RTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEAN
AYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDQAT
SALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVE
KGNHDELMKEKGIYFKLVTMQTVSFWRIMKLNLTEWPYFVVGVFCAIING
GLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFL
QGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDA
AQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAG
VVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQ
SLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFE
DVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYS
TEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSS
GCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPIL
FDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDK
GTQLSGGQKQRIAIARALVRQPHILLLDQATSALDTESEKVVQEALDKAR
EGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVS
VQAG

3D structure

• PDB: 6c0v Molecular structure of human P-glycoprotein in the ATP-bound, outward-facing conformation.
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.1: P-type phospholipid transporter.
  7.6.2.2: ABC-type xenobiotic transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	Y401 R404 S429 G430 G432 K433 S434 T435 Q475 Q1175 S1177 Q1180	Y343 R346 S371 G372 G374 K375 S376 T377 Q417 Q1053 S1055 Q1058
BS02	ATP	A	Q530 S532 Q535 Y1044 T1046 R1047 S1072 G1073 K1076 S1077 T1078 Q1118	Q472 S474 Q477 Y922 T924 R925 S950 G951 K954 S955 T956 Q996
BS03	MG	A	S1077 Q1118	S955 Q996
BS04	MG	A	S434 Q475	S376 Q417

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008559 ABC-type xenobiotic transporter activity
• GO:0015562 efflux transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0022857 transmembrane transporter activity
• GO:0031625 ubiquitin protein ligase binding
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0042910 xenobiotic transmembrane transporter activity
• GO:0046943 carboxylic acid transmembrane transporter activity
• GO:0090554 phosphatidylcholine floppase activity
• GO:0090555 phosphatidylethanolamine flippase activity
• GO:0099038 ceramide floppase activity
• GO:0140326 ATPase-coupled intramembrane lipid transporter activity
• GO:0140328 floppase activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0000086 G2/M transition of mitotic cell cycle
• GO:0006805 xenobiotic metabolic process
• GO:0006869 lipid transport
• GO:0009410 response to xenobiotic stimulus
• GO:0045332 phospholipid translocation
• GO:0046865 terpenoid transport
• GO:0055085 transmembrane transport
• GO:0070633 transepithelial transport
• GO:0072089 stem cell proliferation
• GO:0099040 ceramide translocation
• GO:0140115 export across plasma membrane
• GO:0150104 transport across blood-brain barrier
• GO:1905039 carboxylic acid transmembrane transport
• GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
• GO:1990962 xenobiotic transport across blood-brain barrier
• GO:2001225 regulation of chloride transport

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0009986 cell surface
• GO:0016020 membrane
• GO:0016324 apical plasma membrane
• GO:0070062 extracellular exosome
• GO:0098591 external side of apical plasma membrane

External links

• PDB: RCSB:6c0v, PDBe:6c0v, PDBj:6c0v
• PDBsum: 6c0v
• PubMed: 29371429
• UniProt: P08183|MDR1_HUMAN ATP-dependent translocase ABCB1 (Gene Name=ABCB1)