Structure of PDB 6bs6 Chain A

Receptor sequence

>6bs6A (length=648) Species: 226186 (Bacteroides thetaiotaomicron VPI-5482) [Search protein sequence]

QWTALTASPDTWDETKRADISYQLLLYSFADSDGDGYGDLNGVTQKLDYL
NQLGVKALWLSPIHPCMSYHGYDVTDYTKVNPQLGTESDFDRLVTEAHNR
GIKIYLDYVMNHTGTAHPWFTEASSSSESPYRNYYSFSEDPKTDIAAGKI
AMITQEGAAGYNAAEWFQVSDETAAVKGLLKFTLDWSNAPSPILVVSTGT
KADEDNPDTGTDNAKYLYYGEDICKKFYDKGNNIYELTVDFESTWGLLIR
TSNASFWPSGTKYGASSSSEKLALNKDFKLTNAGNPANIMFDSQQITYFH
SHFCTDWFADLNYGPVDQAGESPAYQAIADAAKGWIARGVDGLRLDAVKH
IYHSETSEENPRFLKMFYEDMNAYYKQKGHTDDFYMIGEVLSEYDKVAPY
YKGLPALFEFSFWYRLEWGINNSTGCYFAKDILSYQQKYANYRSDYIEAT
KLSNHNEDRTSSKLGKSADKCKLAAAVLLTSAGHPYIYYGEELGLYGTKD
NGDEYVRSPMLWGDSYTTNYTDKTDATVSKNVKTVADQQADTHSLLNIYF
SLTRLRNTYPALAEGNMTKHSVYNESQEKDYKPIAAWYMTKDNEKLLVIH
NFGGTAMQLPLTDKIEKVLFVNGETQQNTDSYTLKLGGYASVVFKLGN

3D structure

PDB

6bs6 Structural basis for the flexible recognition of alpha-glucan substrates by Bacteroides thetaiotaomicron SusG.

Chain

Resolution

2.17 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	D149 R386 D388 E431 H497 N498
Catalytic site (residue number reindexed from 1)	D107 R344 D346 E389 H455 N456
Enzyme Commision number	3.2.1.1: alpha-amylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLC	A	W299 K304	W257 K262
BS02	GLC	A	Y260 W287 N330	Y218 W245 N288
BS03	GLC	A	K391 L433	K349 L391
BS04	GLC	A	F345 A389 H392 E431	F303 A347 H350 E389
BS05	GLC	A	Y114 H154 D388 E431 N498	Y72 H112 D346 E389 N456
BS06	GLC	A	H112 Y114 F350 D545 R549	H70 Y72 F308 D503 R507
BS07	GLC	A	S110 H112 D115 T347 W349 F350	S68 H70 D73 T305 W307 F308
BS08	GLC	A	E435 D437	E393 D395
BS09	GLC	A	R457 W460	R415 W418
BS10	GLC	A	Y469 D473	Y427 D431
BS11	CA	A	N153 D352 H392 I393	N111 D310 H350 I351
BS12	CA	A	D73 D75 D77 Y79 D81	D31 D33 D35 Y37 D39

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0004556	alpha-amylase activity
GO:0005509	calcium ion binding
GO:0016798	hydrolase activity, acting on glycosyl bonds
GO:0046872	metal ion binding
GO:2001070	starch binding

	Biological Process
GO:0000272	polysaccharide catabolic process
GO:0005975	carbohydrate metabolic process
GO:0005983	starch catabolic process
GO:0009313	oligosaccharide catabolic process

	Cellular Component
GO:0009279	cell outer membrane
GO:0019867	outer membrane

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:6bs6, PDBe:6bs6, PDBj:6bs6
PDBsum	6bs6
PubMed	29603462
UniProt	Q8A1G3\|SUSG_BACTN Alpha-amylase SusG (Gene Name=susG)

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