Structure of PDB 6brx Chain A

Receptor sequence

>6brxA (length=433) Species: 9606 (Homo sapiens)

EGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQ
LRKAQLQVDRFAMELEQSRNLSNTIVHIDMDAFYAAVEMRDNPELKDKPI
AVGSMSMLSTSNYHARRFGVRAAMPGFIAKRLCPQLIIVPPNFDKYRAVS
KEVKEILADYDPNFMAMSLDEAYLNITKHLEERQNWPEDKRRYFIKNSVV
FGTSAQEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQI
LPNRQAVMDFIKDLPIRKVSGIGKVTEKMLKALGIITCTELYQQRALLSL
LFSETSWHYFLHISLGLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLC
QELCSELAQDLQKERLKGRTVTIKLKNVNFEVKTRASTVSSVVSTAEEIF
AIAKELLKTEIDADFPHPLRLRLMGVRISSFPN

3D structure

• PDB: 6brx Structural Basis for Human DNA Polymerase Kappa to Bypass Cisplatin Intrastrand Cross-Link (Pt-GG) Lesion as an Efficient and Accurate Extender.
• Chain: A
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R63 E199 K321 G356 I357 G358 V360 T361 R454 K468 T469 R470 A471 T473	R35 E171 K236 G271 I272 G273 V275 T276 R369 K383 T384 R385 A386 T388
BS02	dna	A	T44 F49 M133 S134 M135 P153 F155 I156 S388 T390 R413 K414 S415 M416 S417 V418 E419 T421 R507	T16 F21 M105 S106 M107 P125 F127 I128 S303 T305 R328 K329 S330 M331 S332 V333 E334 T336 R422
BS03	dna	A	I165 I166	I137 I138
BS04	MG	A	D107 M108 D198	D79 M80 D170
BS05	MG	A	R352 V354 I357	R267 V269 I272
BS06	MG	A	D107 D198 E199	D79 D170 E171
BS07	0KX	A	D107 M108 A110 F111 Y112 S137 T138 Y141 R144 D198 K328	D79 M80 A82 F83 Y84 S109 T110 Y113 R116 D170 K243

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:6brx, PDBe:6brx, PDBj:6brx
• PDBsum: 6brx
• PubMed: 29715472
• UniProt: Q9UBT6|POLK_HUMAN DNA polymerase kappa (Gene Name=POLK)