Structure of PDB 6bhu Chain A

Receptor sequence

>6bhuA (length=1328) Species: 9913 (Bos taurus)

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAANDPNPCPESSASFLSRITFWWITGMMVQGY
RQPLESTDLWSLNKEDTSEQVVPVLVKNWKKECAKSPSLFKVLYKTFGPY
FLMSFLFKAVHDLMMFAGPEILKLLINFVNDKKAPEWQGYFYTALLFISA
CLQTLVLHQYFHICFVSGMRIKTAVIGAVYRKALVITNAARKSSTVGEIV
NLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMV
LMVPLNAVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAF
KDKVLAIRQEELKVLKKSAYLAAVGTFTWVCTPFLVALSTFAVYVTVDEN
NILDAQKAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRVFLSHED
LDPDSIQRRPIKTNSITVKNATFTWARNDPPTLHGITFSVPEGSLVAVVG
QVGCGKSSLLSALLAEMDKVEGHVTVKGSVAYVPQQAWIQNISLRENILF
GRQLQERYYKAVVEACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSL
ARAVYCDSDVYLLDDPLSAVDAHVGKHIFENVIGPKGLLKNKTRLLVTHA
ISYLPQMDVIIVMSGGKISEMGSYQELLARDGAFAEFLRETWKLVEADKA
QTGQVKLSVYWDYMKAIGLFISFLSIFLFLCNHVASLVSNYWLSLWTDDP
IVNGTQEHTQVRLSVYGALGISQGITVFGYSMAVSIGGIFASRRLHLDLL
HNVLRSPISFFERTPSGNLVNRFSKELDTVDSMIPQVIKMFMGSLFNVIG
ACIIILLATPMAAVIIPPLGLIYFFVQRFYVASSRQLKRLESVSRSPVYS
HFNETLLGVSVIRAFEEQERFIRQSDLKVDENQKAYYPSIVANRWLAVRL
ECVGNCIVLFASLFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSE
METNIVAVERLKEYSETEKEAPWQIQDMAPPKDWPQVGRVEFRDYGLRYR
EDLDLVLKHINVTIDGGEKVGIVGRTGAGKSSLTLGLFRIKESAEGEIII
DDINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSL
ELAHLKGFVSALPDKLNHECAEGGENLSVGQRQLVCLARALLRKTKILVL
DQATAAVDLETDDLIQSTIRTQFDDCTVLTIAHRLNTIMDYTRVIVLDKG
EIQEWGSPSDLLQQRGLFYSMAKDSGLV

3D structure

• PDB: 6bhu ATP Binding Enables Substrate Release from Multidrug Resistance Protein 1.
• Chain: A
• Resolution: 3.14 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.2: ABC-type xenobiotic transporter.
  7.6.2.3: ABC-type glutathione-S-conjugate transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	K416 W653 G681 C682 G683 K684 S685 S686 Q713 E1427 N1428 S1430 G1432	K292 W525 G553 C554 G555 K556 S557 S558 Q585 E1225 N1226 S1228 G1230
BS02	ATP	A	N767 S769 Y1301 V1308 G1329 G1331 K1332 S1333 S1334 Q1374	N639 S641 Y1099 V1106 G1127 G1129 K1130 S1131 S1132 Q1172
BS03	MG	A	S685 Q713	S557 Q585
BS04	MG	A	S1333 Q1374	S1131 Q1172
BS05	CLR	A	M1034 I1038	M832 I836
BS06	CLR	A	I978 F1093 R1130 E1254	I776 F891 R928 E1052
BS07	CLR	A	A481 L599	A357 L475

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008559 ABC-type xenobiotic transporter activity
• GO:0015431 ABC-type glutathione S-conjugate transporter activity
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0022857 transmembrane transporter activity
• GO:0034634 glutathione transmembrane transporter activity
• GO:0042910 xenobiotic transmembrane transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006869 lipid transport
• GO:0009410 response to xenobiotic stimulus
• GO:0034775 glutathione transmembrane transport
• GO:0042908 xenobiotic transport
• GO:0050729 positive regulation of inflammatory response
• GO:0055085 transmembrane transport
• GO:0070729 cyclic nucleotide transport
• GO:0071716 leukotriene transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane

External links

• PDB: RCSB:6bhu, PDBe:6bhu, PDBj:6bhu
• PDBsum: 6bhu
• PubMed: 29290467
• UniProt: Q8HXQ5|MRP1_BOVIN Multidrug resistance-associated protein 1 (Gene Name=ABCC1)