Structure of PDB 6axm Chain A

Receptor sequence
>6axmA (length=340) Species: 100226 (Streptomyces coelicolor A3(2)) [Search protein sequence]
AVPPSLRLPVIEAAFPRQLHPYWPKLQETTRTWLLEKRLMPADKVEEYAD
GLCYTDLMAGYYLGAPDEVLQAIADYSAWYFVWDDRHDRDIVHGRAGAWR
RLRGLLHTALDSPGDHLHHEDTLVAGFADSVRRLYAFLPATWNARFARHF
HTVIEAYDREFHNRTRGIVPGVEEYLELRRLTFAHWIWTDLLEPSSGCEL
PDAVRKHPAYRRAALLSQEFAAWYNDLCSLPKEIAGDEVHNLGISLITHH
SLTLEEAIGEVRRRVEECITEFLAVERDALRFADELADGTVRGKELSGAV
RANVGNMRNWFSSVYWFHHESGRYMVDSWDDRSTPPYVNN
3D structure
PDB6axm Substitution of Aromatic Residues with Polar Residues in the Active Site Pocket of epi-Isozizaene Synthase Leads to the Generation of New Cyclic Sesquiterpenes.
ChainA
Resolution1.8 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y95 F96 D99 D103 R179 R194 F198 N240 S244 K247 E248 G251 F332 H333 R338
Catalytic site (residue number reindexed from 1) Y80 F81 D84 D88 R164 R179 F183 N225 S229 K232 E233 G236 F317 H318 R323
Enzyme Commision number 4.2.3.37: epi-isozizaene synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG A N240 S244 E248 N225 S229 E233
BS02 POP A D99 R194 N240 S244 K247 E248 R338 Y339 D84 R179 N225 S229 K232 E233 R323 Y324
BS03 BTM A L72 Y95 F96 D99 F198 H333 Y339 L57 Y80 F81 D84 F183 H318 Y324
Gene Ontology
Molecular Function
GO:0016829 lyase activity
GO:0046872 metal ion binding
GO:0052680 epi-isozizaene synthase activity

View graph for
Molecular Function
External links
PDB RCSB:6axm, PDBe:6axm, PDBj:6axm
PDBsum6axm
PubMed28967743
UniProtQ9K499|CYC1_STRCO Epi-isozizaene synthase (Gene Name=cyc1)

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