Structure of PDB 6awa Chain A

Receptor sequence

>6awaA (length=475) Species: 160488 (Pseudomonas putida KT2440)

MTQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYTDAEGKLALGGTCL
NVGCIPSKALLDSSWKYKEAKESFNVHGISTGEVKMDVAAMVGRKAGIVK
NLTGGVATLFKANGVTSIQGHGKLLAGKKVEVTKADGTTEVIEAENVILA
SGSRPIDIPPAPVDQNVIVDSTGALEFQAVPKRLGVIGAGVIGLELGSVW
ARLGAEVTVLEALDTFLMAADTAVSKEAQKTLTKQGLDIKLGARVTGSKV
NGNEVEVTYTNAEGEQKITFDKLIVAVGRRPVTTDLLASDSGVTIDERGY
IFVDDYCATSVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKAQMNY
DLIPSVIYTHPEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTG
GFVKVIADAKTDRVLGVHVIGPSAAELVQQGAIAMEFGTSAEDLGMMVFS
HPTLSEALHEAALAVNGGAIHVANR

3D structure

• PDB: 6awa 1.83 Angstrom Resolution Crystal Structure of Dihydrolipoyl Dehydrogenase from Pseudomonas putida in Complex with FAD and Adenosine-5'-monophosphate.
• Chain: A
• Resolution: 1.83 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): L45 C49 C54 S57 V191 E195 F449 H451 E456 N474 R475
• Catalytic site (residue number reindexed from 1): L45 C49 C54 S57 V191 E195 F449 H451 E456 N474 R475
• Enzyme Commision number: 1.8.1.4: dihydrolipoyl dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	A	I187 E211 A212 L213 V245 V277	I187 E211 A212 L213 V245 V277
BS02	FAD	A	I10 G13 P14 G15 E34 K35 T48 C49 G53 C54 K58 H121 G122 S151 G152 I192 R279 G318 D319 M325 L326 A327 Y358	I10 G13 P14 G15 E34 K35 T48 C49 G53 C54 K58 H121 G122 S151 G152 I192 R279 G318 D319 M325 L326 A327 Y358

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004148 dihydrolipoyl dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:6awa, PDBe:6awa, PDBj:6awa
• PDBsum: 6awa
• UniProt: Q88FB1