Structure of PDB 6at2 Chain A

Receptor sequence
>6at2A (length=535) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
GLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGYERGVLTNLGAV
AVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKNDNKPLSPETWQHLKG
LVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSD
EELAGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWY
GNEMKKGMFSMMNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTL
STDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSKEAEPEIYNAIRRD
ALLENVTVREDGTIDFDDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKV
IFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLAGTERGITEPTPTFSA
CFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRA
IIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQ
EKAETLAKLFIDNFDKYTDTPAGAALVAAGPKLHH
3D structure
PDB6at2 Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase.
ChainA
Resolution1.444 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R65 K213 H232 S250 K254 T255 D269 R333
Catalytic site (residue number reindexed from 1) R60 K206 H225 S243 K247 T248 D262 R326
Enzyme Commision number 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A S250 G251 G253 K254 T255 T256 K288 R333 R449 I450 S451 I452 T455 S243 G244 G246 K247 T248 T249 K281 R326 R442 I443 S444 I445 T448
BS02 MN A K213 H232 D269 K206 H225 D262
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
GO:0005509 calcium ion binding
GO:0005524 ATP binding
GO:0016831 carboxy-lyase activity
GO:0017076 purine nucleotide binding
GO:0046872 metal ion binding
Biological Process
GO:0006094 gluconeogenesis
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Cellular Component
External links
PDB RCSB:6at2, PDBe:6at2, PDBj:6at2
PDBsum6at2
PubMed30376300
UniProtP22259|PCKA_ECOLI Phosphoenolpyruvate carboxykinase (ATP) (Gene Name=pckA)

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