Structure of PDB 6asm Chain A

Receptor sequence
>6asmA (length=527) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
LTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGYERGVLTNLGAVA
VDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKNDNKPLSPETWQHLKGL
VTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDE
ELAGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYG
SEMCKGMFSMMNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLS
TDPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDA
LLENVTVREDGTIDFDDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVI
FLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLEPTPTFSACFGAAFLSL
HPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRAIIDAILNGS
LDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQEKAETLAKL
FIDNFDKYTDTPAGAALVAAGPKLHHH
3D structure
PDB6asm Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase.
ChainA
Resolution1.55 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R65 K213 H232 S250 K254 T255 D269 R333
Catalytic site (residue number reindexed from 1) R59 K205 H224 S242 K246 T247 D261 R325
Enzyme Commision number 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A S250 G251 G253 K254 T255 T256 K288 R333 R449 I450 S451 I452 T455 S242 G243 G245 K246 T247 T248 K280 R325 R433 I434 S435 I436 T439
BS02 MN A H232 K254 D269 H224 K246 D261
BS03 THJ A R65 S209 K213 Y286 R333 R59 S201 K205 Y278 R325
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
GO:0005509 calcium ion binding
GO:0005524 ATP binding
GO:0016831 carboxy-lyase activity
GO:0017076 purine nucleotide binding
GO:0046872 metal ion binding
Biological Process
GO:0006094 gluconeogenesis
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:6asm, PDBe:6asm, PDBj:6asm
PDBsum6asm
PubMed30376300
UniProtP22259|PCKA_ECOLI Phosphoenolpyruvate carboxykinase (ATP) (Gene Name=pckA)

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