Structure of PDB 6asi Chain A

Receptor sequence
>6asiA (length=529) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
TPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGYERGVLTNLGAVAV
DTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKNDNKPLSPETWQHLKGLV
TRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEE
LAGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGS
EMKKGMFSMMNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLST
DPKRRLIGDDEHGWDDDGVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDAL
LENVTVREDGTIDFDDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVIF
LTADAFGVLPPVSRLTADQTQYHFLSGFTAKLGITEPTPTFSACFGAAFL
SLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRISIKDTRAIIDAILN
GSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQEKAETLA
KLFIDNFDKYTDTPAGAALVAAGPKLHHH
3D structure
PDB6asi Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase.
ChainA
Resolution1.789 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R65 K213 H232 S250 K254 T255 D269 R333
Catalytic site (residue number reindexed from 1) R58 K204 H223 S241 K245 T246 D260 R324
Enzyme Commision number 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A S250 G251 G253 K254 T255 T256 K288 R333 R449 I450 S451 I452 T455 S241 G242 G244 K245 T246 T247 K279 R324 R435 I436 S437 I438 T441
BS02 MN A K213 H232 D269 K204 H223 D260
BS03 03S A R65 S209 K213 Y286 R333 R58 S200 K204 Y277 R324
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0004611 phosphoenolpyruvate carboxykinase activity
GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
GO:0005509 calcium ion binding
GO:0005524 ATP binding
GO:0016831 carboxy-lyase activity
GO:0017076 purine nucleotide binding
GO:0046872 metal ion binding
Biological Process
GO:0006094 gluconeogenesis
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Cellular Component
External links
PDB RCSB:6asi, PDBe:6asi, PDBj:6asi
PDBsum6asi
PubMed30376300
UniProtP22259|PCKA_ECOLI Phosphoenolpyruvate carboxykinase (ATP) (Gene Name=pckA)

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