Structure of PDB 6as7 Chain A

Receptor sequence

>6as7A (length=870) Species: 9606 (Homo sapiens)

EQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTL
YFLPREMKIDLNTGKETGTPISMKDVYEEFDEKIATKYKIMKFKSKPVEK
NYAFEIPDVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELF
LMNRKIKGPCWLEVKSPQLLNQPVSWCKAEAMALKPDLVNVIKDVSPPPL
VVMAFSMKTMQNAKNHQNEIIAMAALVHHSFALDKAAPKPPFQSHFCVVS
KPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGH
NIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGFGERNATCGRMIC
DVEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYL
LEHTWKDAKFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFL
LLHAFYENNYIVPDKQIFRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLY
PSIIQEFNICFTTVQRVEQIPELPDPSLEMGILPREIRKLVERRKQVKQL
MKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTY
KGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSE
VNKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLD
IVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSV
PVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYV
ICQDGSNLTASQRAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPID
GIDAVLIATWLGLDPTQFRV

3D structure

• PDB: 6as7 Activity and fidelity of human DNA polymerase alpha depend on primer structure.
• Chain: A
• Resolution: 2.95 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	D1002 K1053 Y1055 K1075 G1076 R1081 R1082 T1140	D624 K675 Y677 K697 G698 R703 R704 T762	PDBbind-CN: Kd=58nM
BS02	dna	A	S785 R834 A837 Y839 G841 G842 F962 K1051 R1222	S444 R469 A472 Y474 G476 G477 F584 K673 R844	PDBbind-CN: Kd=58nM
BS03	DCP	A	D860 F861 N862 S863 Y865 K950 N954 T1003 D1004	D495 F496 N497 S498 Y500 K572 N576 T625 D626
BS04	MG	A	D860 F861 D1004	D495 F496 D626
BS05	MG	A	D860 D1004	D495 D626

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:1902975 mitotic DNA replication initiation

External links

• PDB: RCSB:6as7, PDBe:6as7, PDBj:6as7
• PDBsum: 6as7
• PubMed: 29555682
• UniProt: P09884|DPOLA_HUMAN DNA polymerase alpha catalytic subunit (Gene Name=POLA1)