Structure of PDB 5zwk Chain A

Receptor sequence

>5zwkA (length=319) Species: 9606 (Homo sapiens)

DVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYG
IAGVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVC
FDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYAL
YGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYA
KDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPA
NKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPV
ILGSPDDVLEFLKVYEKHS

3D structure

• PDB: 5zwk Location of FBPase catalytic metal binding site: a combined experimental and theoretical study
• Chain: A
• Resolution: 2.104 Å

Enzymatic activity

• Enzyme Commision number: 3.1.3.11: fructose-bisphosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FBP	A	D121 G122 N212 Y215 Y244 G246 M248 Y264 K274 E280	D105 G106 N196 Y199 Y228 G230 M232 Y248 K258 E264
BS02	AMP	A	V17 E20 G21 A24 G26 T27 G28 E29 L30 K112 Y113 V160 M177	V9 E12 G13 A16 G18 T19 G20 E21 L22 K96 Y97 V144 M161
BS03	MG	A	D118 D121 E280	D102 D105 E264
BS04	MG	A	D118 L120	D102 L104

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016787 hydrolase activity
• GO:0016791 phosphatase activity
• GO:0042132 fructose 1,6-bisphosphate 1-phosphatase activity
• GO:0042578 phosphoric ester hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0006094 gluconeogenesis

External links

• PDB: RCSB:5zwk, PDBe:5zwk, PDBj:5zwk
• PDBsum: 5zwk
• UniProt: Q2TU34