Structure of PDB 5zhj Chain A

Receptor sequence
>5zhjA (length=206) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]
MRIDIVTIFPACLDPLRQSLPGKAIESGLVDLNVHDLRRWTHDVHHSVDD
APYGGGPGMVMKAPVWGEALDEICSSETLLIVPTPAGVLFTQATAQRWTT
ESHLVFACGRYEGIDQRVVQDAARRMRVEEVSIGDYVLPGGESAAVVMVE
AVLRLLAGVLGPSYTRPASWRGLDVPEVLLSGDHARIAAWRREVSLQRTR
ERRPDL
3D structure
PDB5zhj Thienopyrimidinone Derivatives That Inhibit Bacterial tRNA (Guanine37-N1)-Methyltransferase (TrmD) by Restructuring the Active Site with a Tyrosine-Flipping Mechanism.
ChainA
Resolution1.75 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) P85 E112 R154
Catalytic site (residue number reindexed from 1) P85 E112 R154
Enzyme Commision number 2.1.1.228: tRNA (guanine(37)-N(1))-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A P83 T84 P85 G109 E112 S132 I133 L138 G140 G141 P83 T84 P85 G109 E112 S132 I133 L138 G140 G141 MOAD: Kd=10.1uM
PDBbind-CN: -logKd/Ki=5.00,Kd=10.1uM
BindingDB: Kd=10100nM
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
GO:0052906 tRNA (guanine(37)-N1)-methyltransferase activity
Biological Process
GO:0002939 tRNA N1-guanine methylation
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0032259 methylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5zhj, PDBe:5zhj, PDBj:5zhj
PDBsum5zhj
PubMed31442049
UniProtP9WFY7|TRMD_MYCTU tRNA (guanine-N(1)-)-methyltransferase (Gene Name=trmD)

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