Structure of PDB 5zcc Chain A

Receptor sequence

>5zccA (length=545) Species: 1409 (Bacillus sp. (in: firmicutes))

KWWKEAVAYQIYPRSFMDSNGDGIGDIQGVISKLDYLSDLGIDVIWICPI
YQSPNDDNGYDISDYKDIMKDFGTMEDFDELLDEVHHRGMKLIMDLVINH
TSDEHPWFLESRSAKENPYRDYYIWHEGKDGKEPNNWESIFSGSAWEFDE
KTKEYYMHVFSKKQPDLNWENEKVRHELYEMVNWWLDKGIDGFRVDAISH
IKKVAGFPDLPNPEKLDYVPSFEGHMNRPGIQEHLKELKEKTFAKYDIMT
VGQANGVTSDSADEWVAEDGGNFNMIFQFEHMGLWKPLDLIELKTILTNW
QNGLEKINGWNALYLENHDQIRSVNKFGSTAYRVESAKCLAALYFLMKGT
PFIYQGQELGMTNVKFDSIDDYDDVGMINYYRIQREKGDSHDEIMKVIWE
TGRDNSRTPMQWNTEKNAGFSTGNPWMKVNPNYVDINVEEQKSDKNSVLN
FYKQLIKIRKQHDVLVYGTYKLLAEEDSAIYAYTRTLEGKTAVVICNMSP
NNQTFEFPSSFTNIEVLIHNYPLDKNETLEQCTLHPYETRVYLIS

3D structure

• PDB: 5zcc Function and structure of GH13_31 alpha-glucosidase with high alpha-(1→4)-glucosidic linkage specificity and transglucosylation activity.
• Chain: A
• Resolution: 1.704 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D98 R197 D199 Q256 H326 D327
• Catalytic site (residue number reindexed from 1): D95 R194 D196 Q253 H318 D319

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	I143 A200 H203 Q256 N258	I140 A197 H200 Q253 N255
BS02	GLC	A	D60 Y63 H103 F163 D199 Q256 H326 D327 R411	D57 Y60 H100 F160 D196 Q253 H318 D319 R403
BS03	CA	A	D21 N23 D25 I27 D29	D18 N20 D22 I24 D26
BS04	CA	A	D534 E537 T543	D524 E527 T533

Gene Ontology

Molecular Function

• GO:0004556 alpha-amylase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0009313 oligosaccharide catabolic process

External links

• PDB: RCSB:5zcc, PDBe:5zcc, PDBj:5zcc
• PDBsum: 5zcc
• PubMed: 29870070
• UniProt: A0A2Z5WH92