Structure of PDB 5z0u Chain A

Receptor sequence

>5z0uA (length=626) Species: 2026 (Thermoactinomyces vulgaris)

AANDNNVEWNGLFHDQGPLFDNAPEPTSTQSVTLKLRTFKGDITSANIKY
WDTADNAFHWVPMVWDSNDPTGTFDYWKGTIPASPSIKYYRFQINDGTST
AWYNGNGPSSTEPNADDFYIIPNFKTPDWLKNGVMYQIFPDRFYNGDSSN
DVQTGSYTYNGTPTEKKAWGSSVYADPGYDNSLVFFGGDLAGIDQKLGYI
KKTLGANILYLNPIFKAPTNHKYDTQDYMAVDPAFGDNSTLQTLINDIHS
TANGPKGYLILDGVFNHTGDSHPWFDKYNNFSSQGAYESQSSPWYNYYTF
YTWPDSYASFLGFNSLPKLNYGNSGSAVRGVIYNNSNSVAKTYLNPPYSV
DGWRLDAAQYVDHQIWSEFRNAVKGVNSNAAIIGEYWGNANPWTAQGNQW
DAATNFDGFTQPVSEWITGKDYQNNSASISTTQFDSWLRGTRANYPTNVQ
QSMMNFLSNHDITRFATRSGGDLWKTYLALIFQMTYVGTPTIYYGDEYGM
QGGADPDNRRSFDWSQATPSNSAVALTQKLITIRNQYPALRTGSFMTLIT
DDTNKIYSYGRFDNVNRIAVVLNNDSVSHTVNVPVWQLSMPNGSTVTDKI
TGHSYTVQNGMVTVAVDGHYGAVLAQ

3D structure

• PDB: 5z0u Mutagenesis-induced conformational change in domain B of a pullulan-hydrolyzing alpha-amylase TVA I
• Chain: A
• Resolution: 1.37 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D262 R354 D356 E396 H471 D472
• Catalytic site (residue number reindexed from 1): D262 R354 D356 E385 H460 D461
• Enzyme Commision number: 3.2.1.135: neopullulanase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	A2 D4 N6 D42 D96	A2 D4 N6 D42 D96
BS02	CA	A	N145 D147 N150 D151 G187 D189	N145 D147 N150 D151 G187 D189
BS03	CA	A	D276 N279 F281 S283 E288	D276 N279 F281 S283 E288

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0031216 neopullulanase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:5z0u, PDBe:5z0u, PDBj:5z0u
• PDBsum: 5z0u
• UniProt: Q60053|NEPU1_THEVU Neopullulanase 1 (Gene Name=tvaI)