Structure of PDB 5yzx Chain A

Receptor sequence
>5yzxA (length=484) Species: 83333 (Escherichia coli K-12) [Search protein sequence]
IDFNDELRNRREKLAALRQQGVAFPNDFRRDHTSDQLHEEFDAKDNQELE
SLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDSLPEGVYNDQ
FKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLDKALRPLPDQEVRYRQ
RYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGAS
ARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISV
RHNPEFTMMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFD
FGKPFEKLTMREAIKKYRPETDMADLDNFDAAKALAESIGITVEKSWGLG
RIVTEIFDEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIG
GREIGNGFSELNDAEDQAERFQEQVNAKAAGDDEAMFYDEDYVTALEYGL
PPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRP
3D structure
PDB5yzx A proposed role for MSC to reserve the canonical function in high eukaryotes prior to stimuli
ChainA
Resolution3.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.6: lysine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 B4P A R262 E264 H270 N271 F274 M276 D376 E421 I422 G477 R480 R244 E246 H252 N253 F256 M258 D358 E403 I404 G459 R462
BS02 CA A E414 E421 N424 E396 E403 N406
BS03 CA A D376 E414 D358 E396
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0003676 nucleic acid binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004824 lysine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006430 lysyl-tRNA aminoacylation
GO:0034605 cellular response to heat
GO:0036260 RNA capping
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5yzx, PDBe:5yzx, PDBj:5yzx
PDBsum5yzx
PubMed
UniProtP0A8N5|SYK2_ECOLI Lysine--tRNA ligase, heat inducible (Gene Name=lysU)

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