Structure of PDB 5xuz Chain A

Receptor sequence

>5xuzA (length=1216) Species: 1410628 (Lachnospiraceae bacterium ND2006)

GSHMSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDY
KGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENL
EINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGF
TTAFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIF
DKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTE
SGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSD
EEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTI
SKDIFGEWNVIRDKWNAEYDDIHLKVVTEKYEDDRRKSFKKIGSFSLEQL
QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKND
AVVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKV
DHIYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYG
SKYYLAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSK
KWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWS
NAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLY
MFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRAS
LKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPI
AINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNI
VEQYSLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELK
AGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKML
IDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWL
TSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYK
NFSRTDADYIKKWKLYSYGNRIRIDWEEVCLTSAYKELFNKYGINYQQGD
IRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGI
FYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVK
IAISNKEWLEYAQTSV

3D structure

• PDB: 5xuz Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1.
• Chain: A
• Resolution: 2.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S14 K15 T16 R18 F19 K20 K51 N157 R158 R174 K253 L261 Q264 K267 Y277 K278 V280 W355 R386 K390 K464 D505 R508 K520 N706 K707 S710 H714 G715 T716 N718 H720 E743 R747 H759 I765 A766 N767 K768 N769 N772 K774 T777 L779 Y781 Y784 K785 D786 R788 Q793 H797 F863 Y872 R887 Q888 E898 G902 S905 H909 K937 K953 K960	S17 K18 T19 R21 F22 K23 K54 N160 R161 R177 K256 L264 Q267 K270 Y280 K281 V283 W358 R386 K390 K464 D505 R508 K520 N706 K707 S710 H714 G715 T716 N718 H720 E743 R747 H759 I765 A766 N767 K768 N769 N772 K774 T777 L779 Y781 Y784 K785 D786 R788 Q793 H797 F863 Y872 R887 Q888 E898 G902 S905 H909 K937 K953 K960
BS02	dna	A	N160 K167 S168 G242 K251 N256 E257 N260 S286 S288 Y290 K349 W355 R508 N509 T512 Q513 K514 D535 K538 Y542 L585 P587 M592 S599 K600 K601 Y646 K648 W649 S739 G740 N895 I896 K897 K945 S982 F983 K984	N163 K170 S171 G245 K254 N259 E260 N263 S289 S291 Y293 K352 W358 R508 N509 T512 Q513 K514 D535 K538 Y542 L585 P587 M592 S599 K600 K601 Y646 K648 W649 S739 G740 N895 I896 K897 K945 S982 F983 K984
BS03	dna	A	K120 K121 G146 F147 T148 T149 K560 K591 K595	K123 K124 G149 F150 T151 T152 K560 K591 K595

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding

External links

• PDB: RCSB:5xuz, PDBe:5xuz, PDBj:5xuz
• PDBsum: 5xuz
• PubMed: 28781234
• UniProt: A0A182DWE3